Fish Interleukin 6,IL-6 ELISA Kit

Fish Interleukin 6,IL-6 ELISA Kit

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中文名称：

鱼白介素6(IL-6)酶联免疫试剂盒
货号：

CSB-E13258Fh
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO鱼类白介素6（IL-6）定量检测专用科研试剂盒（货号：CSB-E13258Fh），采用竞争性酶联免疫吸附法（ELISA）原理，适用于血清、血浆及组织匀浆样本中IL-6的精准检测。IL-6作为重要的细胞因子，在鱼类免疫调节、炎症反应及组织修复过程中发挥关键作用，其浓度变化可反映机体的免疫状态。本试剂盒检测范围为12.5-200 pg/mL，检测下限达到12.5 pg/mL，通过标准品浓度梯度与吸光度值的相关性实现定量分析，可灵敏捕捉样本中微量IL-6的动态变化。该试剂盒为鱼类免疫学研究提供可靠工具，适用于水产养殖中鱼类健康监测、病原感染模型建立、免疫增强剂效果评估等科研场景，亦可用于比较医学中脊椎动物免疫系统的进化机制探究。实验操作全程无需特殊设备，配套标准品、预包被板及显色系统，确保实验高效稳定。本产品严格遵循科研级质量控制标准，所有组分均经多批次验证，保障数据可重复性。
别名：

il6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写：

IL-6
Uniprot No.：

A0S0B0
种属：

Fish
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 pg/mL-200 pg/mL
灵敏度：

12.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	89
	Range %	82-94
1:4	Average %	103
	Range %	94-107
1:8	Average %	96
	Range %	90-101

回收率：

The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	91-104
EDTA plasma (n=4)	91	84-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.128	0.133	0.131
100	0.269	0.258	0.264
50	0.449	0.431	0.440
25	0.851	0.842	0.847
12.5	1.813	1.783	1.798

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2 O Revina,/,2023
Interleukin-6 mediates lipopolysaccharide-inhibited irisin secretion in Nile tilapia (Oreochromis niloticus) Y Yan,Fish & shellfish immunology,2023
Pectin Nanoparticle-Loaded Soft Coral Nephthea sp. Extract as In Situ Gel Enhances Chronic Wound Healing: In Vitro, In Vivo, and In Silico Studies NH Hassan,Pharmaceuticals,2023
Responses of Earthworms to Acetamiprid NO Erhunmwunse,researchsquare,2023
Multiple Biomarker Responses in Female Catfish (Clarias Gariepinus) Exposed to Acetaminophen NO Erhunmwunse,Research Square,2021
Transcriptome analysis of immune-related gene expression in Yellow River carp (Cyprinus carpio var.) after challenge with Flavobacterium columnare Y Jia,Microbial pathogenesis,2021
Dietary taurine modulates hepatic oxidative status, ER stress and inflammation in juvenile turbot (Scophthalmus maximus L.) fed high carbohydrate diets Yue Zhang,Fish & shellfish immunology,2020
Inclusion of condensed tannins in Lateolabrax japonicus diets: Effects on growth, nutrient digestibility, antioxidant and immune capacity and copper sulphate stress resistance K Peng,Aquaculture Reports,2020
The effects of four plant extracts on growth, sex reversal, immunological and haemato‐biochemical parameters in Nile tilapia, Oreochmomis niloticus (Linnaeus, 1758) I Ghosal,Aquaculture Research,2020
Quercetin affects shoaling and anxiety behaviors in zebrafish: Involvement of neuroinflammation and neuron apoptosis J Zhang,Fish & Shellfish Immunology,2020
Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016
Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L.) Modulates Intestinal and Spleen Immune Responses. Magrone T.et al,Oxid Med Cell Longev.,2016

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功能：

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
亚细胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
组织特异性：

Expressed in kidney and spleen. Low expression in liver and gills.

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.