Fish transforming growth factor, beta 1 (TGFB1) ELISA kit

产品详情

产品描述：

CUSABIO鱼类转化生长因子β1（TGFB1）酶联免疫检测试剂盒（货号CSB-EL023446FI），采用双抗体夹心法定量检测鱼类血清、血浆样本中TGFB1的蛋白水平，检测范围覆盖6.25-400 ng/mL。TGFB1作为重要的多功能细胞因子，在鱼类免疫调节、细胞增殖分化及组织修复等生理过程中发挥关键作用，其动态监测对水生生物免疫机制研究具有重要意义。试剂盒包含预包被特异性捕获抗体的酶标板、生物素标记检测抗体及标准品，通过显色反应实现目标蛋白的精准定量，操作流程约需4.5小时完成。该试剂盒适用于科研领域探究环境胁迫、病原感染或营养代谢对鱼类免疫系统的影响，也可用于水产养殖中鱼类健康状态评估及免疫相关分子通路研究，为水生动物免疫学、病理学及生态毒理学研究提供可靠的分析工具。
缩写：

TGFB1
种属：

Fish
样本类型：

serum, plasma
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish TGFB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-95
1:2	Average %	92
	Range %	85-105
1:4	Average %	97
	Range %	90-102
1:8	Average %	95
	Range %	90-105

回收率：

The recovery of fish TGFB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-98
EDTA plasma (n=4)	94	85-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.