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  4. Chicken Transforming Growth factor β1,TGF-β1 ELISA kit

Chicken Transforming Growth factor β1,TGF-β1 ELISA kit

文献引用 (9) 说明书下载
  • 中文名称：
    鸡转化生长因子β1(TGF-β1)酶联免疫试剂盒
  • 货号：
    CSB-E09875Ch
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    本试剂盒专为定量检测鸡血清、血浆及组织匀浆样本中的转化生长因子β1（TGF-β1）设计。TGF-β1是一种多功能细胞因子，在细胞增殖、分化、免疫调节及组织修复等生理过程中发挥重要作用，其动态变化对研究禽类免疫系统调控、炎症反应及生长发育机制具有重要科研价值。采用双抗体夹心酶联免疫吸附法（ELISA），通过特异性抗体对实现0.312-20 ng/mL的线性检测范围，灵敏度达0.078 ng/mL，可精确测定样本中TGF-β1的浓度水平。该试剂盒适用于家禽免疫学基础研究、病理模型建立及生物标志物分析，特别在探究禽类传染性疾病、疫苗效价评估或应激反应机制等科研场景中，能够为实验提供稳定可靠的定量数据支持。试剂盒操作流程标准化，包含预包被96孔板、标准品、检测抗体及显色底物等完整组分，实验全程无需特殊设备，兼容常规实验室条件。
  • 别名：
    TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
  • 缩写：
    TGFB1
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %93
    Range %84-97
    1:2Average %95
    Range %84-98
    1:4Average %96
    Range %89-103
    1:8Average %95
    Range %85-100
  • 回收率：
    The recovery of chicken TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9084-97
    EDTA plasma (n=4)9792-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.011 2.105 2.058 1.965
    101.790 1.828 1.809 1.716
    51.466 1.527 1.497 1.404
    2.51.149 1.163 1.156 1.063
    1.250.795 0.854 0.825 0.732
    0.6250.433 0.425 0.429 0.336
    0.3120.264 0.255 0.260 0.167
    00.095 0.091 0.093 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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TGFB1 antibodies

TGFB1 Proteins

靶点详情

  • 功能：
    Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.; Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.; Transforming growth factor beta-1: Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules'. Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
  • 亚细胞定位：
    [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-1]: Secreted.
  • 蛋白家族：
    TGF-beta family
  • 数据库链接：

    UniGene: Gga.929

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |