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中文名称:鱼补体蛋白3(C3)酶联免疫试剂盒
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货号:CSB-E09727s
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规格:96T/48T
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价格:¥4200/¥3000
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其他:
产品详情
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产品描述:CUSABIO鱼类补体蛋白3(C3)酶联免疫检测试剂盒(货号:CSB-E09727s),采用竞争法定量原理,专为科研领域设计。补体蛋白C3是鱼类先天免疫系统的核心成分,参与调理吞噬、炎症调控及病原清除等过程,其水平变化可反映鱼类免疫状态及感染性疾病的发展动态。该试剂盒适用于血清、血浆样本检测,检测范围覆盖12.5 μg/mL至500 μg/mL,通过预包被特异性抗体与样本中C3蛋白的竞争结合反应实现精准定量,操作流程包含标准品梯度稀释和酶标二抗显色步骤,可在3-4小时内完成检测。该产品广泛应用于水产动物免疫机制研究,如细菌或病毒感染模型中的补体通路分析、养殖环境压力对免疫功能的评估,以及疫苗或免疫增强剂的功效验证等实验场景,为水生生物健康管理和疾病防控研究提供可靠工具。试剂盒组分包含预包被板、标准品、检测抗体及显色底物,需在2-8℃保存并避免反复冻融。
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缩写:
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种属:Fish
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样本类型:serum, plasma
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检测范围:12.5 μg/mL-500 μg/mL
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灵敏度:12.5 μg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Cancer
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 85-100 1:2 Average % 96 Range % 90-108 1:4 Average % 95 Range % 91-98 1:8 Average % 95 Range % 91-100 -
回收率:
The recovery of fish C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 85-94 EDTA plasma (n=4) 95 91-102 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μg/ml OD1 OD2 Average 500 0.176 0.174 0.175 250 0.381 0.393 0.387 115 0.557 0.569 0.563 37.5 0.894 0.901 0.898 12.5 1.481 1.498 1.490 0 2.417 2.542 2.480 -
数据处理:
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货期:3-5 working days
引用文献
- Nano-Bacillus amyloliquefaciens as a dietary intervention in nile tilapia (Oreochromis niloticus): Effects on resistance to Aeromonas hydrophila challenge, immune-antioxidant responses, digestive/absorptive capacity, and growth ME Hassanin, A El-Murr, AR El-Khattib, AA Abdelwarith,Heliyon,2024
- Toxicological insight of metiram: immuno-oxidative, neuro-behavioral, and hemato-biochemical changes during acute exposure of Nile tilapia (Oreochromis niloticus) M Shaalan,BMC veterinary research,2024
- Role of Silver Nanoparticles Synthetized by Marine Red Algae, Acanthophora spicifera, Against Saprolegniosis in Oreochromis niloticus M Sayed,/,2023
- Dietary Artemisia arborescens Supplementation Effects on Growth, Oxidative Status, and Immunity of Gilthead Seabream (Sparus aurata L.) OP Tzortzatos,Animals,2024
- Pseudomonas putida infection induces immune-antioxidant, hepato-renal, ethological, and histopathological/immunohistochemical disruptions in Oreochromis niloticus: the palliative role of titanium dioxide nanogel ANA Rahman,BMC veterinary research,2024
- Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus) RE Ibrahim,/,2024
- The crosstalk between photoperiod and early mild stress on juvenile oscar (Astronotus ocellatus) after acute stress M Zare,Fish physiology and biochemistry,2024
- Effect of dietary intervention with Capsicum annuum extract on growth performance, physiological status, innate immune response, and related gene expression in Nile tilapia RE Ibrahim,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
- The Recovery Time between Early Mild Stress and Final Acute Stress Affects Survival Rate, Immunity, Health, and Physiology of Oscar (Astronotus ocellatus) M Zare,Animals,2023
- Fish Meal Replacement and Early Mild Stress Improve Stress Responsiveness and Survival of Fish after Acute Stress M Zare,Animals,2023
- Early Mild Stress along with Lipid Improves the Stress Responsiveness of Oscar (Astronotus ocellatus) M Esmaeili,Aquaculture Nutrition,2022
- Replacement of dietary fish oil with plant oils improves the immunological responses and the antioxidant status in Oreochromis niloticus exposed to suboptimal temperature RM Reda,Fish Physiology and Biochemistry,2020
- Optimal form of yeast cell wall promotes growth, immunity and disease resistance in gibel carp (Carassius auratus gibelio) P Zhang,Aquaculture Reports,2020
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