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Fish Complement 3,C3 ELISA Kit

  • 中文名称:
    鱼补体蛋白3(C3)酶联免疫试剂盒
  • 货号:
    CSB-E09727s
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    CUSABIO鱼类补体蛋白3(C3)酶联免疫检测试剂盒(货号:CSB-E09727s),采用竞争法定量原理,专为科研领域设计。补体蛋白C3是鱼类先天免疫系统的核心成分,参与调理吞噬、炎症调控及病原清除等过程,其水平变化可反映鱼类免疫状态及感染性疾病的发展动态。该试剂盒适用于血清、血浆样本检测,检测范围覆盖12.5 μg/mL至500 μg/mL,通过预包被特异性抗体与样本中C3蛋白的竞争结合反应实现精准定量,操作流程包含标准品梯度稀释和酶标二抗显色步骤,可在3-4小时内完成检测。该产品广泛应用于水产动物免疫机制研究,如细菌或病毒感染模型中的补体通路分析、养殖环境压力对免疫功能的评估,以及疫苗或免疫增强剂的功效验证等实验场景,为水生生物健康管理和疾病防控研究提供可靠工具。试剂盒组分包含预包被板、标准品、检测抗体及显色底物,需在2-8℃保存并避免反复冻融。
  • 缩写:
  • 种属:
    Fish
  • 样本类型:
    serum, plasma
  • 检测范围:
    12.5 μg/mL-500 μg/mL
  • 灵敏度:
    12.5 μg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cancer
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 85-100  
    1:2 Average % 96  
    Range % 90-108  
    1:4 Average % 95  
    Range % 91-98  
    1:8 Average % 95  
    Range % 91-100  
  • 回收率:
    The recovery of fish C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-94  
    EDTA plasma (n=4) 95 91-102  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    500 0.176 0.174 0.175    
    250 0.381 0.393 0.387    
    115 0.557 0.569 0.563    
    37.5 0.894 0.901 0.898    
    12.5 1.481 1.498 1.490    
    0 2.417 2.542 2.480    
  • 数据处理:
  • 货期:
    3-5 working days

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