Dog Complement 4(C4) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO犬补体蛋白4（C4）酶联免疫检测试剂盒（货号：CSB-EQ027970DO），采用竞争法原理实现定量检测，适用于科研领域对犬血清、血浆及组织匀浆样本中C4蛋白水平的分析。补体蛋白C4是补体系统经典途径和凝集素途径的关键成分，参与抗体介导的免疫清除和炎症反应调控，其浓度变化可为研究犬类免疫相关疾病机制、感染性疾病进程或自身免疫异常提供重要依据。试剂盒检测灵敏度高，线性检测范围覆盖31.25-2000 ng/mL，通过预包被特异性抗体与样本中C4蛋白竞争结合标记物实现精准定量，实验流程优化后可在常规实验室条件下完成操作。该试剂盒支持多种生物样本检测，适用于动物模型研究、免疫系统功能评估、药物干预效果观测等科研场景，为犬类基础医学研究提供标准化工具。实验所需96孔板及标准品、检测抗体等核心组分均经严格验证，确保数据稳定性和重复性，满足科研工作者对补体系统相关分子定量分析的需求。
缩写：

C4
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 ng/mL-2000 ng/mL
灵敏度：

7.8 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-105
1:400	Average %	96
	Range %	92-99
1:800	Average %	103
	Range %	98-109
1:1600	Average %	87
	Range %	82-91

回收率：

The recovery of dog C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-98
EDTA plasma (n=4)	90	87-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.