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  4. Rat Complement 4,C4 ELISA Kit

Rat Complement 4,C4 ELISA Kit

文献引用 (3) 说明书下载
  • 中文名称：
    大鼠补体蛋白4(C4)酶联免疫试剂盒
  • 货号：
    CSB-E08706r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠补体蛋白4（C4）酶联免疫检测试剂盒（货号：CSB-E08706r），基于双抗体夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中C4蛋白的精准分析。补体蛋白C4是补体系统的关键成分，在先天免疫应答中通过参与经典和凝集素途径的级联反应，调控病原体清除及炎症反应，其表达水平的变化常被用于探索免疫调节机制及炎症相关疾病的病理过程。试剂盒检测灵敏度高，线性范围覆盖12.5 ng/mL至800 ng/mL，可满足不同样本浓度需求。实验操作便捷，用户可通过标准曲线实现样本中C4含量的定量计算。该试剂盒适用于体外免疫学研究，如炎症动物模型中的血清C4动态监测、组织局部补体激活水平评估，或药物干预下补体通路的功能研究，为免疫学、病理学及药理学领域的科研工作提供可靠工具。所有实验需遵循标准操作规范以确保数据准确性。
  • 别名：
    C4 ELISA Kit; C4aComplement C4 [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain] ELISA Kit
  • 缩写：
    C4
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 ng/mL-800 ng/mL
  • 灵敏度：
    3.12 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %99
    Range %96-102
    1:400Average %87
    Range %82-93
    1:800Average %100
    Range %96-104
    1:1600Average %84
    Range %81-89
  • 回收率：
    The recovery of rat C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9390-96
    EDTA plasma (n=4)8884-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8002.604 2.658 2.631 2.540
    4002.185 2.198 2.192 2.101
    2001.673 1.698 1.686 1.595
    1001.069 1.097 1.083 0.992
    500.626 0.636 0.631 0.540
    250.292 0.271 0.282 0.191
    12.50.188 0.180 0.184 0.093
    00.090 0.092 0.091
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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C4 Proteins

靶点详情

  • 功能：
    Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes.; Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory processes. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • 基因功能参考文献：
    1. Our study provides the first evidence that sustained selective EPAC activation leads to an increase in TRPC3 and C4 protein expression and induces a proarrhythmic SOCE-like activity in adult rat ventricular cardiomyocytes PMID: 26219954
    2. The expression of the complement component C3f and complement component 4A have potential for use in post-transplant diagnosis of rejection. PMID: 23613499
    3. This study presents the genomic organization of the two C4 gene-containing modules and the expression of the two C4 genes in the rat (Rattus norvegicus). PMID: 15634920
  • 亚细胞定位：
    Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
  • 数据库链接：

    KEGG: rno:24233

    STRING: 10116.ENSRNOP00000037902

    UniGene: Rn.155573

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |