Dog Complement 3(C3)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬补体蛋白3(C3)酶联免疫试剂盒（货号：CSB-EL003888DO）专为科研领域设计，用于定量检测犬血清、血浆及组织匀浆样本中的补体蛋白C3水平。C3是补体系统的核心成分，参与调理吞噬、免疫复合物清除等先天免疫反应，其水平变化与炎症、感染及自身免疫疾病密切相关，是犬类免疫机制研究的重要靶点。CUSABIO试剂盒采用竞争性酶联免疫吸附法（ELISA）原理，检测范围覆盖0.78-50 μg/mL，灵敏度可精确至0.78 μg/mL，操作流程包含标准品梯度稀释及样本预处理步骤，试剂组分需按说明书保存（短期4°C，长期-20°C），有效期6个月。该产品适用于实验动物模型研究、免疫病理机制探索及药物干预效果评价等科研场景，例如通过检测感染性疾病或免疫相关疾病模型中C3的浓度变化，揭示补体系统在犬类疾病发生发展中的作用，为兽医学研究提供可靠的数据支持。
别名：

AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
缩写：

C3
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 μg/mL-50 μg/mL
灵敏度：

0.195 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	95-102
1:200	Average %	95
	Range %	81-89
1:400	Average %	92
	Range %	88-96
1:800	Average %	101
	Range %	98-103

回收率：

The recovery of dog C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-97
EDTA plasma (n=4)	89	85-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.