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Chicken Tumor necrosis factor α,TNF-α ELISA KIT

  • 中文名称:
    鸡肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
  • 货号:
    CSB-E11231Ch
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

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  • 产品描述:
    CUSABIO试剂盒采用竞争法原理定量检测鸡血清、血浆及组织匀浆样本中的肿瘤坏死因子α(TNF-α),该因子是禽类免疫调节的核心细胞因子,在宿主抵抗病毒/细菌感染、自身免疫性疾病及肿瘤发生等病理生理过程中起关键作用。产品货号为CSB-E11231Ch,检测灵敏度达0.27 pg/mL,线性检测范围为0.27-200 pg/mL,可精准量化微量生物样本中的TNF-α浓度。适用于科研领域研究禽类传染性疾病(如禽流感、新城疫)的炎症反应机制,评估疫苗或抗炎药物对免疫系统的调控效果,以及构建家禽疾病模型时监测炎症因子动态变化。试剂盒配套预包被抗体与标准品,支持96孔板批量检测,孵育流程经优化实现3.5小时内完成实验,为实验室提供高灵敏度、高特异性的检测方案,是开展禽类免疫学研究的重要工具。
  • 别名:
    DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2, APC1 protein|TNF superfamily, member 2|TNF, macrophage-derived|TNF, monocyte-derived|cachectin|tumor necrosis factor alpha
  • 缩写:
    TNF-α
  • 种属:
    Gallus gallus (Chicken)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.27 pg/mL-200 pg/mL
  • 灵敏度:
    0.27 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-96  
    1:2 Average % 100  
    Range % 95-105  
    1:4 Average % 87  
    Range % 82-91  
    1:8 Average % 93  
    Range % 86-98  
  • 回收率:
    The recovery of chicken TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 93-108  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    200 0.173 0.170 0.172    
    66.7 0.259 0.251 0.255    
    22.2 0.347 0.367 0.357    
    7.41 0.452 0.488 0.470    
    2.47 0.611 0.633 0.622    
    0.82 0.809 0.855 0.832    
    0.27 1.033 1.018 1.026    
    0 1.748 1.707 1.728    
  • 数据处理:
  • 货期:
    3-5 working days

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靶点详情

  • 最新研究进展:
    TNF-α(肿瘤坏死因子-α)是一种促炎症蛋白质,它在多种疾病中扮演重要角色。最新研究表明,TNF-α可能参与COVID-19的发病机制,因为这种病毒通过激活NF-κB途径来增加TNF-α的表达,从而引发炎症反应和危及生命的并发症。此外,TNF-α也参与了许多自身免疫疾病的发生和发展过程,例如风湿性关节炎和肠道炎症性疾病。