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中文名称:鱼肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
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货号:CSB-E13254Fh
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规格:96T/48T
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价格:¥4200/¥3000
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其他:
产品详情
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产品描述:鱼肿瘤坏死因子α(TNF-α)酶联免疫试剂盒(CSB-E13254Fh)为竞争法ELISA试剂盒,定量检测血清、血浆样本中的TNF-α含量。TNF-α,即肿瘤坏死因子-α,是一种重要的细胞因子,主要由活化的巨噬细胞产生。它通过与其受体TNFR1结合,激活多种信号通路,影响细胞增殖、凋亡和炎症反应。TNF-α在多种疾病如炎症、自身免疫性疾病中起关键作用,其研究有助于开发新的治疗策略。试剂盒检测范围为125 pg/mL-5000 pg/mL,本试剂盒适用于鱼类免疫相关研究,如病原感染模型中的炎症反应监测、疫苗免疫效果评估、水产养殖动物健康状态分析等科研场景,为水生生物免疫学机制研究及水产疾病防控提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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缩写:TNF-α
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Uniprot No.:
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种属:Fish
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样本类型:serum, plasma
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检测范围:125 pg/mL-5000 pg/mL
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灵敏度:125 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 89-100 1:2 Average % 93 Range % 87-99 1:4 Average % 95 Range % 88-103 1:8 Average % 96 Range % 90-102 -
回收率:
The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 95-102 EDTA plasma (n=4) 95 91-101 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average 5000 0.154 0.156 0.155 2500 0.351 0.328 0.340 1150 0.562 0.535 0.549 375 0.861 0.864 0.863 125 1.544 1.530 1.537 0 2.387 2.355 2.371 -
数据处理:
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货期:3-5 working days
引用文献
- TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2 O Revina,/,2023
- Irisin inhibits CCK-8-induced TNF-α production via integrin αVβ5-NF-κB signaling pathways in Nile tilapia (Oreochromis niloticus) W Deng,Fish & shellfish immunology,2023
- Pectin Nanoparticle-Loaded Soft Coral Nephthea sp. Extract as In Situ Gel Enhances Chronic Wound Healing: In Vitro, In Vivo, and In Silico Studies NH Hassan,Pharmaceuticals,2023
- Molecular characterization and immune functions of lipasin in Nile tilapia (Oreochromis niloticus): Involvement in the regulation of tumor necrosis factor-α secretion R Dong,Fish & shellfish immunology,2023
- Transcriptome analysis of immune-related gene expression in Yellow River carp (Cyprinus carpio var.) after challenge with Flavobacterium columnare Y Jia,Microbial pathogenesis,2021
- Dietary taurine modulates hepatic oxidative status, ER stress and inflammation in juvenile turbot (Scophthalmus maximus L.) fed high carbohydrate diets Yue Zhang,Fish & shellfish immunology,2020
- Inclusion of condensed tannins in Lateolabrax japonicus diets: Effects on growth, nutrient digestibility, antioxidant and immune capacity and copper sulphate stress resistance K Peng,Aquaculture Reports,2020
- Quercetin affects shoaling and anxiety behaviors in zebrafish: Involvement of neuroinflammation and neuron apoptosis J Zhang,Fish & Shellfish Immunology,2020
- Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
- Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016
- Cytokine Responses in Gills of Capoeta umbla as Biomarkers of Environmental Pollution Danabas D.et al,Water Environ Res.,2016
靶点详情
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功能:Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
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亚细胞定位:Membrane; Single-pass type II membrane protein.
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蛋白家族:Tumor necrosis factor family
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