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Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT

  • 中文名称:
    鱼肿瘤坏死因子α(TNF-α)酶联免疫试剂盒
  • 货号:
    CSB-E13254Fh
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    鱼肿瘤坏死因子α(TNF-α)酶联免疫试剂盒(CSB-E13254Fh)为竞争法ELISA试剂盒,定量检测血清、血浆样本中的TNF-α含量。TNF-α,即肿瘤坏死因子-α,是一种重要的细胞因子,主要由活化的巨噬细胞产生。它通过与其受体TNFR1结合,激活多种信号通路,影响细胞增殖、凋亡和炎症反应。TNF-α在多种疾病如炎症、自身免疫性疾病中起关键作用,其研究有助于开发新的治疗策略。试剂盒检测范围为125 pg/mL-5000 pg/mL,本试剂盒适用于鱼类免疫相关研究,如病原感染模型中的炎症反应监测、疫苗免疫效果评估、水产养殖动物健康状态分析等科研场景,为水生生物免疫学机制研究及水产疾病防控提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    TNF-α
  • Uniprot No.:
  • 种属:
    Fish
  • 样本类型:
    serum, plasma
  • 检测范围:
    125 pg/mL-5000 pg/mL
  • 灵敏度:
    125 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 89-100  
    1:2 Average % 93  
    Range % 87-99  
    1:4 Average % 95  
    Range % 88-103  
    1:8 Average % 96  
    Range % 90-102  
  • 回收率:
    The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 95-102  
    EDTA plasma (n=4) 95 91-101  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    5000 0.154 0.156 0.155    
    2500 0.351 0.328 0.340    
    1150 0.562 0.535 0.549    
    375 0.861 0.864 0.863    
    125 1.544 1.530 1.537    
    0 2.387 2.355 2.371    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能:
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
  • 亚细胞定位:
    Membrane; Single-pass type II membrane protein.
  • 蛋白家族:
    Tumor necrosis factor family