Bovine Fibrinogen,Fb Elisa Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛纤维蛋白原（Fibrinogen, Fb）酶联免疫检测试剂盒（货号：CSB-E13283B），采用竞争法定量原理，适用于血清、血浆样本中纤维蛋白原含量的精准分析。纤维蛋白原是参与凝血过程的关键蛋白，在牛类动物的止血机制、炎症反应及组织修复研究中具有重要价值。试剂盒检测灵敏度高，线性范围为3.125-200 μg/mL，可覆盖常见生理及病理状态下的浓度变化需求。实验操作简便，需使用预包被的96孔板，通过标准品与样本中目标蛋白竞争结合特异性抗体，结合显色反应实现定量检测。试剂盒包含标准品、检测抗体、显色液及终止液等完整组分，开封前4℃保存有效期达6个月，实验全程需在4小时内完成，建议样本避免反复冻融以保证检测准确性。本产品适用于动物医学研究、畜牧业健康监测、营养代谢评估等科研领域，特别针对凝血功能异常模型或炎症相关研究中的纤维蛋白原动态变化分析，为科研人员提供稳定、可重复的检测方案。
别名：

N/A
缩写：

Fb
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma
检测范围：

3.125 μg/mL-200 μg/mL
灵敏度：

1.56 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine Fb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	90
	Range %	86-97
1:40	Average %	96
	Range %	92-100
1:80	Average %	85
	Range %	81-86
1:160	Average %	89
	Range %	83-98

回收率：

The recovery of bovine Fb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	96	92-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.