Human Fibrinogen(FB) ELISA Kit

Human Fibrinogen(FB) ELISA Kit

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中文名称：

人纤维蛋白原（Fb）酶联免疫试剂盒
货号：

CSB-E13656h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本试剂盒（货号：CSB-E13656h）采用双抗体夹心法原理，专为定量检测人血清、血浆或组织匀浆样本中的纤维蛋白原（Fibrinogen, Fb）设计。纤维蛋白原作为肝脏合成的重要凝血因子，不仅参与止血和血栓形成过程，其浓度变化还与心血管疾病、炎症反应及组织修复等病理生理机制密切相关。试剂盒检测范围覆盖0.312-20 μg/mL，能够精准捕捉生理状态至疾病模型中的浓度波动，满足体外实验中对低丰度样本和高浓度样本的检测需求。实验操作中通过预包被特异性抗体捕获目标蛋白，结合酶标二抗显色系统实现定量分析，确保检测的高特异性和稳定性。该产品适用于基础医学研究中凝血/纤溶系统调控机制探索、肝脏功能评估、心血管疾病动物模型验证，以及炎症相关病理过程的分子机制研究，为体外药物筛选、疾病模型构建和生物标志物分析提供可靠工具。
别名：

N/A
缩写：

Fb
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 μg/mL-20 μg/mL
灵敏度：

0.078 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	85
	Range %	80-92
1:200	Average %	98
	Range %	90-105
1:400	Average %	97
	Range %	90-105
1:800	Average %	93
	Range %	86-98

回收率：

The recovery of human FB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	98	90-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average	Corrected
20	2.648	2.701	2.675	2.583
10	2.159	2.169	2.164	2.072
5	1.476	1.536	1.506	1.414
2.5	0.864	0.876	0.870	0.778
1.25	0.443	0.475	0.459	0.367
0.625	0.234	0.253	0.244	0.152
0.312	0.146	0.154	0.150	0.058
0	0.095	0.089	0.092

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

The activation of complement C5a-C5aR1 axis in astrocytes facilitates the neuropathogenesis due to EV-A71 infection by upregulating CXCL1 P Zhu, W Ji, D Li, F Wang, T Sun, H Yang,Journal of Virology,2024
Calcium Chloride vs. Mechanical Preparation of Fibrinogen-Depleted Human Platelet Lysate: Implications for Umbilical Cord Mesenchymal Stem Cell Culture YT Lim, M Barathan, YL Tan, YT Lee, JX Law,Life,2024
Preparation of Fibrinogen-Depleted Human Platelet Lysate to Support Heparin-Free Expansion of Umbilical Cord-Derived Mesenchymal Stem Cells LT Kee,Journal of translational medicine,2023
Identification and characterization of novel mutations in Chinese patients with congenital fibrinogen disorders P Zhou,Blood Cells, Molecules, and Diseases,2020
Lower level of complement component C3 and C3a in the plasma means poor outcome in the patients with hepatitis B virus related acute-on-chronic liver … Q Li,BMC Gastroenterol,2020
Identification and Modulation of Microenvironment is Crucial for Effective MSC Therapy in Acute Lung Injury Islam D, et al,American journal of respiratory and critical care medicine,2018
A novel fibrinogen gamma-chain mutation, p. Cys165Arg, causes disruption of the γ165Cys-Bβ227Cys disulfide bond and ultimately leads to hypofibrinogenemia WJ Zhou, et al,Thrombosis Research,2018
Two symptomatic cases of dysfibrinogenemia in China: one with gamma-chain Arg275Cys mutation and another without detectable mutation in fibrinogen genes. Bin Q et al,Blood Coagul Fibrinolysis,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.