CTCF
CTCF，即CCCTC结合因子，别名11-zinc finger protein、CTCFL paralog。CTCF是一种多功能转录因子，含有11个高度保守的锌指结构域，能够与DNA结合并发挥功能，如介导募集cohesin、参与X染色体失活、异染色质边界的形成、V(D)J重组、RNA剪切、凋亡等。CTCF通过识别特定的DNA序列基序结合到染色质上，并作为锚点固定染色质环，是构建染色质三维结构的基础。CTCF在细胞生长与增殖、系统发生以及肿瘤发生等方面都有着重要的作用。CTCF的缺失会导致多种细胞分化和组织发育缺陷，包括影响记忆和学习能力、心血管发育、血液细胞分化以及肢端发育等。CTCF通过建立细胞谱系特异染色质高级结构调控决定细胞命运基因表达的分子机制，在多种生物学程序中发挥关键作用。
热销产品
Recombinant Human Transcriptional repressor CTCF (CTCF), partial (CSB-CF006138HU1)
验证数据
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
CTCF Antibody (CSB-PA006138DSR2HU)
验证数据
Western blot
All lanes: Transcriptional repressor CTCF antibody at 6μg/ml
Lane 1: PC-3 whole cell lysate
Lane 2: MCF-7 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 83, 46 kDa
Observed band size: 83 kDa
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA006138DSR2HU at dilution of 1:100
Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-CTCF or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP006138HU) against the H19ICR promoter.
CTCF Antibodies
CTCF for Homo sapiens (Human)
| 产品货号 | 产品名称 | 种属反应性 | 应用类型 |
|---|---|---|---|
| CSB-PA006138GA01HU | CTCF Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA006138DSR2HU | CTCF Antibody | Human | ELISA, WB, IHC, ChIP |
| CSB-PA006138DSR1HU | CTCF Antibody | Human | ELISA, IHC |
CTCF Proteins
CTCF Proteins for Homo sapiens (Human)
| 产品货号 | 产品名称 | 来源 |
|---|---|---|
| CSB-YP006138HU1 CSB-EP006138HU1 CSB-BP006138HU1 CSB-MP006138HU1 CSB-EP006138HU1-B |
Recombinant Human Transcriptional repressor CTCF (CTCF), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-CF006138HU1 | Recombinant Human Transcriptional repressor CTCF (CTCF), partial | in vitro E.coli expression system |
CTCF Proteins for Gallus gallus (Chicken)
| 产品货号 | 产品名称 | 来源 |
|---|---|---|
| CSB-YP600891CH CSB-EP600891CH CSB-BP600891CH CSB-MP600891CH CSB-EP600891CH-B |
Recombinant Chicken Transcriptional repressor CTCF (CTCF), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTCF Proteins for Mus musculus (Mouse)
| 产品货号 | 产品名称 | 来源 |
|---|---|---|
| CSB-YP726738MO1 CSB-EP726738MO1 CSB-BP726738MO1 CSB-MP726738MO1 CSB-EP726738MO1-B |
Recombinant Mouse Transcriptional repressor CTCF (Ctcf), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CTCF Proteins for Rattus norvegicus (Rat)
| 产品货号 | 产品名称 | 来源 |
|---|---|---|
| CSB-YP874175RA CSB-EP874175RA CSB-BP874175RA CSB-MP874175RA CSB-EP874175RA-B |
Recombinant Rat Transcriptional repressor CTCF (Ctcf) | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
