CD276

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized CD276 at 2 μg/ml can bind Anti-CD276 rabbit monoclonal antibody, the EC₅₀ of human CD276 protein is 1.961-2.243 ng/ml.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD276 at 2 μg/ml can bind Anti-CD276 recombinant antibody(CSB-RA733578MA1HU). The EC₅₀ is 4.299-5.373 ng/mL.

The purity of CD276 was greater than 90% as determined by SEC-HPLC

The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP5140MOV) at 2 μg/mL can bind Anti-CD276 recombinant antibody. The EC₅₀ is 4.299-5.373 ng/mL.

The Binding Activity of Human CD276 with Anti-CD276 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized human CD276(CSB-MP733578HU(F2)) at 2 μg/mL can bind Anti-CD276 recombinant antibody. The EC₅₀ is 47.12-54.16 ng/mL.

Overlay Peak curve showing MCF7 cells stained with CSB-RA733578MA1HUMA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.