Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

文献引用 (8) 说明书下载
  • 中文名称：
    大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶联免疫试剂盒
  • 货号：
    CSB-E07443r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶联免疫试剂盒（CSB-E07443r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、尿液样本中的NAG含量。大鼠N-乙酰-β-D-氨基葡萄糖苷酶（NAG）是一种溶酶体水解酶，主要存在于近端肾小管中，与黏多糖类及糖蛋白代谢相关。其活性变化与肾小管损伤密切相关，可作为肾损伤的早期标志物。目前，NAG的研究主要集中在其生物学机制、靶点以及与肾小管疾病的关系上。试剂盒检测范围为1.56 mIU/mL-100 mIU/mL，该产品可广泛应用于肾脏疾病机制研究、药物肾毒性评估、代谢综合征模型构建等科研领域，尤其适用于动物实验中对尿液或血液样本的NAG动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
  • 缩写：
    NAG
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, urine
  • 检测范围：
    1.56 mIU/mL-100 mIU/mL
  • 灵敏度：
    0.39 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %96
    Range %91-102
    1:4Average %87
    Range %84-95
    1:8Average %95
    Range %92-101
  • 回收率：
    The recovery of rat NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-105
    EDTA plasma (n=4)9690-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    1002.495 2.471 2.483 2.306
    501.955 1.987 1.971 1.794
    251.356 1.368 1.362 1.185
    12.50.934 0.963 0.949 0.772
    6.250.587 0.597 0.592 0.415
    3.120.425 0.451 0.438 0.261
    1.560.361 0.374 0.368 0.191
    00.175 0.178 0.177 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

显示更多
收起更多

产品评价

相关产品

Hexb Proteins

靶点详情

  • 功能：
    Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
  • 亚细胞定位：
    Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
  • 蛋白家族：
    Glycosyl hydrolase 20 family
  • 数据库链接：

    KEGG: rno:294673

    UniGene: Rn.203067

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |