Rat Carcinoembryonic Antign,CEA ELISA Kit

产品详情

产品描述：

大鼠癌胚抗原(CEA)酶联免疫试剂盒（CSB-E13926r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CEA含量。癌胚抗原（CEA）是一种存在于粘膜细胞中的糖蛋白，正常情况下在婴幼儿和成人粘膜细胞中存在。在癌症患者中，癌胚抗原的表达会增加，因此常作为肿瘤标志物。CEA的研究机制涉及其在细胞粘附、免疫反应、失巢凋亡和肝转移中的作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，癌症机制研究、实验动物模型中的肿瘤进展监测、体外研究肿瘤微环境、评估抗癌药物干预效果、筛选潜在生物标志物、肿瘤生物学研究、药理毒理实验、基础医学探索本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

CEA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	86	80-89

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.