Rat 6-keto-PGF1α ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠6-酮前列腺素F1α(6-keto-PGF1α)酶联免疫试剂盒（CSB-E14411r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、组织培养上清液样本中的6-keto-PGF1α含量。6-keto-PGF1α是一种由PGI2代谢生成的非活性产物，作为PGI2生物合成的有用标志物。其研究机制涉及HIF-1α、TXB2、6-Keto-PGF1α和HA的联合检测，以反映肝脏微循环障碍状况，其在慢性乙型肝炎等疾病诊断中具有重要意义。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，常用于研究血管舒缩调节、炎症反应及血栓形成等生理病理过程；支持心血管疾病模型、药物干预实验及炎症机制等基础研究，尤其适用于探索氧化应激、内皮损伤或抗血小板药物药效评价等领域；通过检测不同样本中6 - keto - PGF1α的动态变化，可为血管功能相关科研课题提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

6-keto-PGF1α,6-keto-PGF1 alpha
缩写：

6-keto-PGF1α
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat 6-keto-PGF1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	88
	Range %	82-95
1:4	Average %	90
	Range %	85-95
1:8	Average %	92
	Range %	84-97

回收率：

The recovery of rat 6-keto-PGF1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-100
EDTA plasma (n=4)	98	93-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.