Rabbit follicle-stimulating hormone,FSH ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔子促卵泡素(FSH)酶联免疫试剂盒（CSB-E06918Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FSH含量。FSH即促卵泡生成素，它在生殖生理中扮演关键角色，调控卵泡发育和精子生成。其研究机制围绕与受体结合，激活下游信号通路，影响生殖细胞的增殖分化。对FSH深入研究有助于理解生殖内分泌疾病，为相关疾病诊断和治疗提供理论支撑。试剂盒检测范围为1.56 mIU/mL-100 mIU/mL，该产品适用于科研领域中对兔子生殖内分泌系统的机制研究、繁殖调控实验评估或药物对性腺功能影响的体外分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

FSHBFollitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
缩写：

FSH
Uniprot No.：

Q6IY73
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 mIU/mL-100 mIU/mL
灵敏度：

0.39 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	99
	Range %	91-105
1:4	Average %	103
	Range %	92-110
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
数据库链接：

KEGG: ocu:100008946

STRING: 9986.ENSOCUP00000016404

UniGene: Ocu.3174