Rabbit Interleukin 18,IL-18 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔子白介素18(IL-18)酶联免疫试剂盒（CSB-E13942Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IL-18含量。IL-18是一个重要靶点。它属于白细胞介素家族，在免疫调节中发挥关键作用。研究发现，IL-18参与多种炎症相关疾病发生发展。其作用机制主要是通过结合受体激活下游信号通路，调控免疫细胞功能，为相关疾病治疗提供了新方向。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研场景中IL - 18浓度的精准分析，例如免疫机制研究、炎症动物模型评估或疫苗开发中免疫原性评价。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

IL-18
Uniprot No.：

B1A3U4
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rabbit IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	102	92-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.