Pig neutrophil gelatinase-associated lipocalin(NGAL)ELISA Kit

产品详情

产品描述：

猪中性粒细胞明胶酶相关脂质运载蛋白(NGAL)酶联免疫试剂盒（CSB-E16507p）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NGAL含量。试剂盒检测范围为7.8 pg/mL-500 pg/mL，可广泛应用于猪模型中的炎症应答机制研究、肾功能损伤评估及药物干预效果分析等领域，能为兽医病理学、畜牧科学及比较医学领域的科研工作者提供可靠工具，助力于动物疾病模型的建立与新型治疗策略开发。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

NGAL
Uniprot No.：

F1RRX1
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	105
	Range %	101-108
1:400	Average %	96
	Range %	92-98
1:800	Average %	96
	Range %	90-99
1:1600	Average %	89
	Range %	84-93

回收率：

The recovery of pig NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-94
EDTA plasma (n=4)	99	99-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.