Pig Interleukin 18,IL-18 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪白介素18(IL-18)酶联免疫试剂盒（CSB-E06781p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL18含量。IL18 即白细胞介素 18，是一种多功能促炎细胞因子，在先天免疫和获得性免疫中均发挥重要作用。其研究机制主要围绕它能诱导 T 细胞和自然杀伤细胞产生γ-干扰素，增强自然杀伤细胞的细胞毒活性，在炎症、免疫相关疾病研究中备受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研场景中动态监测实验动物模型（如细菌/病毒感染、免疫调节研究）的免疫应答水平；为兽医学、比较免疫学及动物疾病模型研究提供可靠工具，助力科研人员解析IL - 18在猪免疫系统中的分子机制与调控网络。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：

IL18
Uniprot No.：

O19073
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-92
1:2	Average %	100
	Range %	95-105
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
基因功能参考文献：
1. Study reports that the monocyte-derived cytokines IL-12 and IL-18 act synergistically on porcine gammadelta T cells to induce IFN-gamma production. Additional stimuli such as the mitogen ConA and IL-2 are necessary to activate the cells for enhanced proliferation. PMID: 25036760
2. IL-18 concentration in saliva was significantly increased during a 60-min acute immobilization stress in thirteen 5-month-old pigs. These results are the first evidence of a stress-related change of IL-18 in pig saliva. PMID: 21682650
3. endometrial expression of CASP1 and IL18 associated with pregnancy establishment; alteration of CASP1 and IL18 following premature exposure of uterus to estrogen during early pregnancy may contribute to conceptus loss between Days 15 to 18 of pregnancy PMID: 20380728
4. Porcine IL-18 regulates anti-pig cellular rejection in C57BL/6 mice. PMID: 17079874
亚细胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
数据库链接：

KEGG: ssc:397057

STRING: 9823.ENSSSCP00000015962

UniGene: Ssc.20