Mouse carcinoembryonic antign,CEA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠癌胚抗原(CEA)酶联免疫试剂盒（CSB-E13925m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CEACAM5含量。CEACAM5是一种重要靶点。它在多种恶性肿瘤中高表达，与肿瘤的发生、发展、转移等密切相关。研究机制主要聚焦于其对肿瘤细胞增殖、黏附、侵袭等生物学行为的影响，科学家也在探索基于此靶点开发针对性的抗癌药物及疗法。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，本产品在小鼠模型中的动态监测对癌症机制研究、治疗药物筛选及疗效评估具有重要意义，适用于肿瘤模型构建、基因编辑动物验证、肿瘤微环境分析等科研场景，助力肿瘤生物学、免疫治疗及转化医学等领域的实验探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Ceacam5 ELISA Kit; Psg30Carcinoembryonic antigen-related cell adhesion molecule 5 ELISA Kit; Pregnancy-specific glycoprotein 30 ELISA Kit
缩写：

CEACAM5
Uniprot No.：

Q3UKK2
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CEA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-93
1:2	Average %	98
	Range %	95-100
1:4	Average %	102
	Range %	97-107
1:8	Average %	94
	Range %	91-96

回收率：

The recovery of mouse CEA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-105
EDTA plasma (n=4)	93	90-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.
基因功能参考文献：
1. a new mechanistic insight into how the CEA IgV-like N domain participates in cellular events that can have a macroscopic impact in terms of cancer progression and metastasis. PMID: 24388361
亚细胞定位：

Cell membrane; Lipid-anchor, GPI-anchor. Apical cell membrane. Cell surface.
蛋白家族：

Immunoglobulin superfamily, CEA family
数据库链接：

KEGG: mmu:73250

UniGene: Mm.244138