Mouse Retinoic Acid ELISA kit

• An assay plate --- The 96-well plate has been pre-coated with the anti-mouse retinoic acid antibody.
• Lyophilized standard (1 x 200 μl) --- Reconstitute the standard with sample diluent at dilution series and draw the standard curve.
• HRP-conjugate retinoic acid (100 x concentrate) (1 x 60 μl)--- HRP catalyzes the TMB substrate for color development and needs to be diluted before use.
• HRP-avidin Diluent (1 x 10 ml) --- Dilute the HRP-avidin.
• Sample Diluent (2 x 20 ml) --- Reconstitute the standard and dilute the sample to an appropriate concentration.
• Wash Buffer (25 x concentrate) (1 x 20 ml) --- Wash away the unbound solution and non-specific substances.
• TMB Substrate --- TMB interacts with HRP, eliciting the solution turns blue.
• Stop Solution (1 x 10 ml) --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
• Four Adhesive Strips (For 96 wells) --- Cover the microtiter plate when incubation.
• An instruction manual

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• A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
• An incubator that can provide stable incubation conditions up to 37°C±5°C.
• Centrifuge
• Vortex
• Squirt bottle, manifold dispenser, or automated microplate washer
• Absorbent paper for blotting the microtiter plate
• 50-300ul multi-channel micropipette
• Pipette tips
• Single-channel micropipette with different ranges
• 100ml and 500ml graduated cylinders
• Deionized or distilled water
• Timer
• Test tubes for dilution

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