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中文名称：小鼠8羟基脱氧鸟苷(8-OHdG)酶联免疫试剂盒
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货号：CSB-E10527m
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规格：96T/48T
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价格：￥3800/￥2500
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其他：
产品详情
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产品描述：CUSABIO小鼠8羟基脱氧鸟苷（8-OHdG）酶联免疫检测试剂盒（货号：CSB-E10527m），采用竞争法测定原理，可对血清、血浆及组织匀浆样本中的8-OHdG进行定量分析。8-OHdG是DNA氧化损伤的重要生物标志物，其水平可反映机体氧化应激状态，常用于评估自由基对遗传物质的损伤程度及抗氧化干预效果。该试剂盒检测灵敏度高，线性范围为0.78-50 pg/mL，最低检测限达0.78 pg/mL，能够精准检测微量样本中8-OHdG的动态变化。实验操作基于竞争性结合原理，通过生物素标记物与样本中目标分子的竞争反应实现定量检测，结果稳定可靠。适用于神经退行性疾病模型、衰老机制研究、环境毒理学评估及代谢性疾病等科研领域，可为氧化损伤相关机制研究、药物筛选及抗氧化剂功效评价提供数据支持，广泛应用于基础医学、药理学及生物化学等科研场景。
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缩写：8-OHdG
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种属：Mus musculus (Mouse)
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样本类型：serum, plasma, tissue homogenates
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检测范围：0.78 pg/mL-50 pg/mL
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灵敏度：0.195 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Cell Biology
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse 8-OHdG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 90 Range % 87-97 1:200 Average % 97 Range % 93-104 1:400 Average % 85 Range % 82-91 1:800 Average % 90 Range % 85-97 -
回收率：
The recovery of mouse 8-OHdG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 85-93 EDTA plasma (n=4) 92 88-94 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 50 0.071 0.074 0.073 25 0.120 0.130 0.125 12.5 0.200 0.206 0.203 6.25 0.335 0.340 0.338 3.12 0.573 0.554 0.564 1.56 0.858 0.841 0.850 0.78 1.129 1.139 1.134 0 2.215 2.185 2.200 -
数据处理：
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货期：3-5 working days
引用文献
- Afamin Ameliorates Testosterone Propionate (TP)-Induced Oxidative Stress and Mitochondrial Damage in Human Ovarian Granulosa Tumor Cells (KGN) by Upregulating the Expression of SIRT1 YL Ma, ZW Li, Z Wang, AJ Yang,Molecular Biology,2024
- Fisetin exerts neuroprotective effects in vivo and in vitro by inhibiting ferroptosis and oxidative stress after traumatic brain injury H Yang, Y Hong, M Gong, S Cai, Z Yuan,Frontiers in Pharmacology,2024
- Myricanol prevents aging‐related sarcopenia by rescuing mitochondrial dysfunction via targeting peroxiredoxin 5 S Shen,MedComm,2024
- Temporal analysis of lung injury induced by real‐ambient PM2.5 exposure in mice H Zeng,Environmental toxicology,2023
- Alleviation of ischemia-reperfusion induced renal injury by chemically modified SOD2 mRNA delivered via lipid nanoparticles Y Hou,Molecular therapy. Nucleic acids,2023
- Panax notoginseng saponins prevent dementia and oxidative stress in brains of SAMP8 mice by enhancing mitophagy Y Yang,BMC complementary medicine and therapies,2024
- Apigenin alleviates cancer drug Sorafenib induced multiple toxic effects in Swiss albino mice via anti-oxidative stress Deepti Singh,Toxicology and Applied Pharmacology,2023
- Controlled Release of Hydrogen‐Carrying Perfluorocarbons for Ischemia Myocardium‐Targeting 19F MRI‐Guided Reperfusion Injury Therapy C Nie,Advanced Science,2023
- Neuroinflammation, Oxidative Stress, Apoptosis, Microgliosis and Astrogliosis in the Cerebellum of Mice Chronically Exposed to Waterpipe Smoke N Hamadi,Biomedicines,2023
- Implication of Lupeol in compensating Sorafenib-induced perturbations of redox homeostasis: A preclinical study in mouse model H Fatma,Life sciences,2023
- Rosiglitazone reduces diabetes angiopathy by inhibiting mitochondrial dysfunction dependent on regulating HSP22 expression L Yu,iScience,2023
- Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia P García-Rodríguez,Nutrients,2022
- Circular RNA circ_Cabin1 promotes DNA damage in multiple mouse organs via inhibition of non-homologous end-joining repair upon PM2. 5 exposure H Zeng,Archives of toxicology,2021
- The mechanism of CaMK2α-MCU-mitochondrial oxidative stress in bupivacaine-induced neurotoxicity Z Liu,Free Radical Biology and Medicine,2020
- Rosmarinic Acid, the Main Effective Constituent of Orthosiphon stamineus, Inhibits Intestinal Epithelial Apoptosis Via Regulation of the Nrf2 Pathway in Mice Cai X, et al,Molecules,2019
- Renoprotective effects of brown adipose tissue activation in diabetic mice Cai YY,Journal of Diabetes,2019
- HSP22 suppresses diabetes-induced endothelial injury by inhibiting mitochondrial reactive oxygen species formation LingligYu, et al,Redox Biology,2019
- Lentivirus-Mediated HDAC3 Inhibition Attenuates Oxidative Stress in APPswe/PS1dE9 Mice Yu L.et al,J Alzheimers Dis,2018
- RA-XII exerts anti-oxidant and anti-inflammatory activities on lipopolysaccharide-induced acute renal injury by suppressing NF-魏B and MAPKs regulated by HO-1/Nrf2 An X.et al,Biochem Biophys Res Commun,2017
- Klotho ameliorates cyclosporine A?induced nephropathy via PDLIM2/NF-kB p65 signaling pathway Meihua Jin.et al,Biochemical and Biophysical Research Communications,2017
- Effects of methyl mercury on the activity and gene expression of mouse Langerhans islets and glucose metabolism. Maqbool F.et al,Food Chem Toxicol.,2016
- The Protective Effects of Melatonin Against Oxidative Stress and Inflammation Induced by Acute Cadmium Exposure in Mice Testis Li R. et al,Biol Trace Elem Res,2015
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