Human Platelet-Derived Growth Factor AA(PDGF-AA) ELISA kit

产品详情

产品描述：

人血小板衍生生长因子AA(PDGF-AA)酶联免疫试剂盒（CSB-E17143h）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-AA含量。PDGF - AA 即血小板衍生生长因子 AA 型，是一种重要的细胞因子。它在细胞增殖、分化等过程中发挥关键作用，与组织修复、血管生成等生理过程相关。研究机制主要围绕其与受体结合，激活下游信号通路，在肿瘤、纤维化等疾病发病机制中有重要研究价值。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于心血管疾病机制研究、组织再生模型分析、抗纤维化药物开发等科研领域；为探索 PDGF - AA 在炎症反应、胚胎发育或创伤愈合中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

PDGF-AA
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-AA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	90-102
1:2	Average %	100
	Range %	92-104
1:4	Average %	92
	Range %	85-96
1:8	Average %	94
	Range %	85-99

回收率：

The recovery of human PDGF-AA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	104	95-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.