Fish Acetylcholinesterase(AChE)ELISA Kit

产品详情

产品描述：

鱼乙酰胆碱酯酶(AChE)酶联免疫试剂盒（CSB-E17001Fh）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AChE含量。AChE即乙酰胆碱酯酶，是一种重要的神经递质水解酶。背景上，它在神经系统中对维持神经冲动正常传导至关重要。研究机制方面，主要聚焦于其如何高效水解乙酰胆碱，以调控神经信号传递，相关研究可为神经系统疾病治疗药物研发提供方向。试剂盒检测范围为2.5 ng/mL-40 ng/mL，该产品适用于科研领域的环境毒理学研究，例如水体污染物对鱼类神经功能的影响评估、生态毒性机制探究或实验室条件下鱼类模型的神经生化指标监测，为水产养殖安全、环境污染生物标志物筛选等研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

acheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
缩写：

AChE
Uniprot No.：

P04058
种属：

Fish
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.5 ng/mL-40 ng/mL
灵敏度：

2.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-103
1:2	Average %	92
	Range %	87-101
1:4	Average %	93
	Range %	87-100
1:8	Average %	89
	Range %	83-99

回收率：

The recovery of fish AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
亚细胞定位：

[Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.; [Isoform T]: Cell membrane; Peripheral membrane protein. Cell junction, synapse. Note=Attached to the membrane through disulfide linkage with the collagenic subunit, itself bound to the membrane.
蛋白家族：

Type-B carboxylesterase/lipase family
组织特异性：

Found in the synapses and to a lower extent in extrajunctional areas of muscle and nerve, and on erythrocyte membranes.