Dog Interleukin 18,IL-18 ELISA KIT

Dog Interleukin 18,IL-18 ELISA KIT

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中文名称：

犬白介素18(IL-18)酶联免疫试剂盒
货号：

CSB-E09848c
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

犬白介素18(IL-18)酶联免疫试剂盒（CSB-E09848c）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL18含量。IL18是一种细胞因子，属于白细胞介素家族。它在免疫反应中发挥重要作用，能调节Th1、NK等细胞的活性。研究机制上，IL18可通过与受体结合激活下游信号通路，参与炎症反应和免疫调节，其异常表达与多种疾病相关，是药物研发的潜在靶点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研领域对犬类炎症性疾病（如败血症、自身免疫病）、肿瘤免疫微环境或疫苗免疫效果的研究，支持多类型样本同步检测，为动物实验中的免疫指标分析提供标准化工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：

IL18
Uniprot No.：

Q9XSR0
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-109
1:2	Average %	97
	Range %	91-105
1:4	Average %	90
	Range %	85-94
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of dog IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	87	80-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.675	2.552	2.614	2.439
400	2.065	2.076	2.071	1.896
200	1.372	1.424	1.398	1.223
100	0.726	0.739	0.733	0.558
50	0.471	0.504	0.488	0.313
25	0.380	0.359	0.370	0.195
12.5	0.271	0.259	0.265	0.090
0	0.173	0.177	0.175

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
亚细胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
数据库链接：

KEGG: cfa:403796

STRING: 9615.ENSCAFP00000038185

UniGene: Cfa.40

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.