Bovine Interleukin-18(IL18) ELISA kit

中文名称：

牛白介素18(IL18)酶联免疫试剂盒
货号：

CSB-EL011608BO
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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牛白介素18(IL18)酶联免疫试剂盒（CSB-EL011608BO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL18含量。IL-18是一种促炎细胞因子，在先天性和适应性免疫反应中发挥关键作用。它可诱导Th1细胞产生IFN-γ，参与免疫调节。多个研究表明，IL-18在多种炎症性疾病、自身免疫病和肿瘤的发病机制中起重要作用，是极具潜力的治疗靶点。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于研究牛感染性疾病模型（如病毒或细菌感染）、免疫调节机制探索、疫苗效力评估等科研场景，也可用于评估营养干预或环境应激对牛免疫状态的影响。通过标准化操作流程，研究者可快速获得IL18在免疫相关研究中的动态变化数据，为深入解析牛类免疫系统功能提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：

IL18
Uniprot No.：

Q9TU73
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	95
	Range %	92-99
1:8	Average %	93
	Range %	87-98

回收率：

The recovery of bovine IL18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-110
EDTA plasma (n=4)	97	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	3.011	2.978	2.995	2.900
1000	2.511	2.522	2.517	2.422
500	1.496	1.504	1.500	1.405
250	0.705	0.716	0.711	0.616
125	0.382	0.375	0.379	0.284
62.5	0.226	0.234	0.230	0.135
31.25	0.152	0.159	0.156	0.061
0	0.093	0.097	0.095

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Effects of Lonicera japonica Extract with Different Contents of Chlorogenic Acid on Lactation Performance, Serum Parameters, and Rumen Fermentation in Heat-Stressed Holstein High-Yielding Dairy Cows F Ma,Animals,2024
Haemato-chemical and immune variations in Holstein cows at different stages of lactation, parity, and age SB Kim,CAAS Agricultural Journals,2020

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功能：

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
基因功能参考文献：
1. These results suggest that BAPC-1 is a stem/progenitor cell line and modulates the immuno-endocrine function of the anterior pituitary cells through its production of interleukin-18 and the IL-18 receptor. PMID: 18761716
亚细胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
数据库链接：

KEGG: bta:281249

STRING: 9913.ENSBTAP00000055793

UniGene: Bt.234

Bovine Interleukin-18(IL18) ELISA kit

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