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  4. Rat Aspartate Aminotransferase (AST) ELISA kit

Rat Aspartate Aminotransferase (AST) ELISA kit

文献引用 (15) 说明书下载
  • 中文名称：
    大鼠天门冬氨酸氨基转移酶(AST)酶联免疫试剂盒
  • 货号：
    CSB-E13023r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠天门冬氨酸氨基转移酶(AST)酶联免疫试剂盒（CSB-E13023r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的GOT1含量。GOT1是一种重要靶点。它参与细胞内氨基酸代谢，在细胞能量生成和生物合成中起关键作用。研究发现其异常表达与多种疾病尤其是肿瘤发生发展相关，解析其相关机制可助力开发以GOT1为靶点的新型治疗药物和策略。试剂盒检测范围为3.12 mIU/mL-200 mIU/mL，在肝脏病理机制研究、心肌功能实验及药物毒性评估等科研场景中具有重要应用价值；适用于体外实验中的组织损伤模型评估、生化指标动态监测及相关分子机制探索，为疾病模型构建、药效学评价等基础研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Got1Aspartate aminotransferase ELISA kit; cytoplasmic ELISA kit; cAspAT ELISA kit; EC 2.6.1.1 ELISA kit; EC 2.6.1.3 ELISA kit; Cysteine aminotransferase ELISA kit; cytoplasmic ELISA kit; Cysteine transaminase ELISA kit; cytoplasmic ELISA kit; cCAT ELISA kit; Glutamate oxaloacetate transaminase 1 ELISA kit; Transaminase A ELISA kit
  • 缩写：
    GOT1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    3.12 mIU/mL-200 mIU/mL
  • 灵敏度：
    0.78 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-92
    1:2Average %104
    Range %100-107
    1:4Average %93
    Range %88-97
    1:8Average %98
    Range %92-103
  • 回收率：
    The recovery of rat AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9591-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    2002.094 2.068 2.081 1.918
    1001.276 1.310 1.293 1.130
    500.838 0.784 0.811 0.648
    250.521 0.539 0.530 0.367
    12.50.353 0.334 0.344 0.181
    6.250.300 0.288 0.294 0.131
    3.120.241 0.237 0.239 0.076
    00.164 0.162 0.163 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

Kindly address the below queries:
1. The standard ALT enzyme provided in the kit is from which source ?
2. The Standard ALT enzyme given is active or inactive ?
3. Since we cannot collect more than 150 µL of blood from mice, we can use only 50 µL serum for ELISA. Will it fall within the detectable range ?

A:
We are glad to inform you the following details:
CSB-E13023r,Rat Aspartate Aminotransferase (AST) ELISA kit
Standard:293 cell-derived recombinant protein
Sorry we did not do the activity analysis on the standard.
As to the sample loading volume, we suggest you refer to the manual/datasheet of these 2 kits. If the sample volume is too little, the target might not be recognized during the assay.
If any further questions,pls let me know.
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靶点详情

  • 功能：
    Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
  • 基因功能参考文献：
    1. Hydrogen sulfide (H2 S) is synthesized in peripheral neurons through two mitochondrial enzymes, mercaptopyruvate sulfurtransferase (MPST)and cysteine aminotransferase (CAT). PMID: 24611772
    2. In vivo lentiviral gene delivery of GOT minimized lesion volume, whereas GOT knockdown worsened stroke outcomes. Thus, brain tissue GOT emerges as a novel target in managing stroke outcomes. PMID: 21361730
    3. Results suggest that downregulation of the endogenous SO(2)/GOT pathway is likely involved in the pathogenesis of ISO-induced myocardial injury. PMID: 20733562
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 组织特异性：
    Expressed in liver and kidney.
  • 数据库链接：

    KEGG: rno:24401

    STRING: 10116.ENSRNOP00000022309

    UniGene: Rn.5819

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |