Rat Tryptophan 5-hydroxylase 2(TPH2) ELISA kit

中文名称：

大鼠色氨酸5羟化酶2(TPH2)酶联免疫试剂盒
货号：

CSB-EL024101RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO大鼠色氨酸5羟化酶2（TPH2）酶联免疫检测试剂盒（货号：CSB-EL024101RA），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中TPH2的蛋白表达水平。TPH2是催化色氨酸转化为5-羟色胺（5-HT）的关键限速酶，主要分布于中枢神经系统，其表达水平与神经递质代谢、情绪调节及神经精神疾病机制研究密切相关。试剂盒检测灵敏度高，线性范围为31.25-2000 pg/mL，最低检测限为7.81 pg/mL，可覆盖不同样本浓度的检测需求。预包被特异性抗体可高效捕获目标蛋白，通过优化实验体系确保数据稳定性和重复性，适用于神经科学、精神疾病模型研究或药物干预实验中TPH2的动态监测，例如探究抑郁症、焦虑症等疾病模型中5-HT系统的调控机制，或评估神经活性药物对TPH2表达的影响。试剂盒包含标准品、检测抗体及显色底物等完整组分，操作流程标准化，兼容常规酶标仪进行吸光度分析，为科研人员提供便捷高效的检测方案。
别名：

Tph2 ELISA Kit; Ntph ELISA Kit; Tryptophan 5-hydroxylase 2 ELISA Kit; EC 1.14.16.4 ELISA Kit; Neuronal tryptophan hydroxylase ELISA Kit; Tryptophan 5-monooxygenase 2 ELISA Kit
缩写：

TPH2
Uniprot No.：

Q8CGU9
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TPH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-102
1:2	Average %	94
	Range %	87-98
1:4	Average %	102
	Range %	96-105
1:8	Average %	91
	Range %	87-95

回收率：

The recovery of rat TPH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	97	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.123	2.262	2.193	2.092
1000	1.495	1.585	1.540	1.439
500	1.012	1.044	1.028	0.927
250	0.664	0.677	0.671	0.570
125	0.442	0.451	0.447	0.346
62.5	0.276	0.289	0.283	0.182
31.25	0.191	0.195	0.193	0.092
0	0.102	0.100	0.101

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Rat Tryptophan 5-hydroxylase 2(TPH2) ELISA kit

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