Rabbit Soluble protein-100B,S-100B ELISA Kit

中文名称：

兔子S100B蛋白(S-100B)酶联免疫试剂盒
货号：

CSB-E06895Rb
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO定量检测兔血清、血浆及组织匀浆样本中S100B蛋白的酶联免疫试剂盒，采用双抗体夹心法原理，检测灵敏度达31.25 pg/mL，线性范围为31.25-2000 pg/mL。S100B蛋白属于钙结合蛋白家族，主要由神经胶质细胞和施万细胞表达，在神经发育、细胞增殖及损伤修复中发挥重要作用，其异常表达与神经系统疾病、脑损伤及肿瘤微环境调控等机制相关。本试剂盒包含预包被特异性抗体的96孔板、标准品及检测所需全套试剂，支持科研人员高效分析不同样本中S100B的浓度动态，适用于神经退行性疾病模型研究、脑损伤动物实验、神经胶质瘤机制探索等基础研究领域。实验操作全程约需4小时，仅需微量样本（50-100 μL）即可完成检测，配套标准曲线拟合工具可快速实现数据定量分析，为实验室开展神经生物学、细胞应激反应等研究提供可靠工具。
别名：

S100B ELISA Kit; Protein S100-B ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 calcium-binding protein B ELISA Kit
缩写：

S100B
Uniprot No.：

Q6YNR6
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	94
	Range %	90-99
1:4	Average %	103
	Range %	99-110
1:8	Average %	93
	Range %	89-98

回收率：

The recovery of rabbit S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	92-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.505	2.652	2.579	2.480
1000	1.968	1.976	1.972	1.873
500	1.274	1.204	1.239	1.140
250	0.713	0.739	0.726	0.627
125	0.436	0.424	0.430	0.331
62.5	0.251	0.259	0.255	0.156
31.25	0.182	0.185	0.184	0.085
0	0.097	0.101	0.099

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
基因功能参考文献：
1. The time course of S100B serum values following spinal cord decompression correlates with outcome; the initial degree of paresis is not a prognostic factor to predict outcome. PMID: 21535942
亚细胞定位：

Cytoplasm. Nucleus.
蛋白家族：

S-100 family
数据库链接：

KEGG: ocu:100009495

STRING: 9986.ENSOCUP00000001267

UniGene: Ocu.2593

Rabbit Soluble protein-100B,S-100B ELISA Kit

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