Dog S100 calcium binding protein B (S100B) ELISA kit

产品详情

产品描述：

CUSABIO犬S100B蛋白（S100 Calcium Binding Protein B）定量检测试剂盒，采用双抗体夹心法原理，适用于检测血清、血浆及组织匀浆样本中的S100B蛋白浓度。S100B蛋白是一种钙结合蛋白，主要在中枢神经系统的胶质细胞中表达，参与调控细胞周期、神经生长及损伤修复过程，其浓度变化与神经系统疾病、脑损伤等病理状态密切相关。本试剂盒检测范围为39-2500 pg/mL，通过预包被高亲和力抗体实现精准捕获靶蛋白，配合HRP标记检测抗体完成特异性识别，显色信号与样本浓度呈正相关。样本处理流程简便，无需复杂预处理即可直接用于检测，实验全程可在4小时内完成。该产品适用于神经科学基础研究、疾病模型评估及药物开发等科研场景，例如通过检测外周血或脑组织样本中的S100B水平，探究犬类神经退行性病变机制或创伤性脑损伤的生物标志物动态变化，为动物实验提供可靠的定量分析工具。
缩写：

S100B
Uniprot No.：

E2QTP3
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

39 pg/mL-2500 pg/mL
灵敏度：

9.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig S100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-91
1:2	Average %	93
	Range %	87-98
1:4	Average %	102
	Range %	98-106
1:8	Average %	98
	Range %	94-103

回收率：

The recovery of pig S100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	89	84-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.