Horse growth hormone,GH ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

马生长激素(GH)酶联免疫试剂盒（CSB-E11242Eq）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GH1含量。GH1 为生长激素 1 基因，其表达产物生长激素与人体生长发育密切相关。研究机制方面，它主要通过与受体结合，激活多种信号通路，促进细胞增殖、分化和蛋白质合成。异常的 GH1 表达或功能可能引发如巨人症、侏儒症等生长异常疾病。试剂盒检测范围为25 ng/mL-400 ng/mL，适用于科研领域探究马匹生长调控、营养代谢、繁殖性能及疾病模型中的GH动态变化。适用于实验室对马科动物生理病理机制研究、激素相关药物开发评估以及畜牧领域GH水平监测等科研需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
缩写：

GH1
Uniprot No.：

P01245
种属：

Equus caballus (Horse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

25 ng/mL-400 ng/mL
灵敏度：

25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	87
	Range %	84-90
1:4	Average %	96
	Range %	90-102
1:8	Average %	90
	Range %	85-96

回收率：

The recovery of horse GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-98
EDTA plasma (n=4)	96	91-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能参考文献：
1. Plasma levels of growth hormone (as well as glucose, insulin, and leptin) are highly correlated with the duration of winter anovulatory phase. PMID: 21958120
亚细胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
数据库链接：

KEGG: ecb:100034180

STRING: 9796.ENSECAP00000007142

UniGene: Eca.12604