Sheep C-reactive protein(CRP) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

绵羊C反应蛋白(CRP)酶联免疫试剂盒（CSB-E15081Sh）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CRP含量。CRP（C反应蛋白）是一种由肝脏合成的蛋白质，当体内发生炎症时，CRP水平会升高。它作为炎症标志物，能结合肺炎链球菌等病原体，激活补体系统，增强白细胞吞噬作用，参与非特异性免疫反应。试剂盒检测范围为0.625 μg/mL-40 μg/mL，适用于绵羊模型中炎症机制研究、疾病进程评估及免疫调控相关科研实验，例如感染性疾病模型构建中CRP动态监测、应激反应评估或药物干预效果分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

CRP
Uniprot No.：

W5PD71
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.625 μg/mL-40 μg/mL
灵敏度：

0.156 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	96
	Range %	92-100
1:4	Average %	92
	Range %	87-96
1:8	Average %	95
	Range %	87-98

回收率：

The recovery of sheep CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	90	84-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.