Pig Cystatin C,Cys-C ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪半胱氨酸蛋白酶抑制剂/胱抑素C（Cys-C）酶联免疫试剂盒（货号：CSB-E13282p），基于竞争性酶联免疫吸附原理开发，适用于血清、血浆及组织匀浆样本中Cys-C的定量检测。Cys-C是一种内源性半胱氨酸蛋白酶抑制剂，广泛参与细胞内外蛋白水解调控，在肾脏代谢研究中具有重要生物学意义。该试剂盒检测灵敏度达0.78 μg/mL，线性范围为0.78-50 μg/mL，通过特异性抗体实现目标蛋白的高效捕获与定量分析，实验流程标准化且重复性良好。其配套试剂包含预包被板、标准品及显色系统，支持科研人员开展猪模型相关研究，如肾脏功能评估、代谢性疾病机制探索或药物干预效果验证等方向，尤其适用于动物实验样本中Cys-C表达水平的动态监测，为畜牧兽医学、比较医学及基础病理学研究提供可靠工具。本产品仅限科研使用，操作时需参照说明书进行样本预处理与浓度换算。
缩写：

CST3
Uniprot No.：

Q0Z8R0
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 μg/mL-50 μg/mL
灵敏度：

0.2 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	93
	Range %	89-97
1:4	Average %	92
	Range %	84-96
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of pig Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	9-103
EDTA plasma (n=4)	93	89-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.