Pig coagulation factor VIII(FVIII) ELISA Kit

Pig coagulation factor VIII(FVIII) ELISA Kit

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中文名称：

猪凝血因子VIII(FVIII)酶联免疫试剂盒
货号：

CSB-E15000p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

猪凝血因子VIII(FVIII)酶联免疫试剂盒（CSB-E15000p）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F8含量。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，适用于出血性疾病机制研究、凝血调节药物开发、动物模型凝血功能评估等科研领域，特别适用于畜牧科学、兽医学研究及生物制药领域的凝血相关实验分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

F8 ELISA kit; CF8Coagulation factor VIII ELISA kit; Procoagulant component ELISA kit
缩写：

F8
Uniprot No.：

P12263
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 ng/mL-4000 ng/mL
灵敏度：

15.62 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FVIII in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	89
	Range %	84-95
1:200	Average %	94
	Range %	89-99
1:400	Average %	93
	Range %	86-100
1:800	Average %	98
	Range %	91-103

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

4000

0.134

0.128

0.131

2000

0.221

0.237

0.229

1000

0.384

0.398

0.391

500

0.615

0.629

0.622

250

0.868

0.881

0.875

125

1.214

1.253

1.234

62.5

1.649

1.684

1.667

2.236

2.125

2.181

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
基因功能参考文献：
1. It was concluded that VEGF and factor VIII are important growth factors associated with fetal development in pigs and are identified in all uterine segments. PMID: 28237345
2. Report Factor VIII organisation on nanodiscs with different lipid composition. PMID: 25589466
3. thrombin stimulates transglutaminase activity in articular cartilage by directly cleaving factor XIII and by receptor-mediated up-regulation of factor XIII synthesis PMID: 14983233
4. cupredoxin-like A1 subdomains in fVIII contain inter-species differences that are a result of selective pressure on the dissociation rate constant PMID: 16513639
亚细胞定位：

Secreted, extracellular space.
蛋白家族：

Multicopper oxidase family
数据库链接：

KEGG: ssc:397339

STRING: 9823.ENSSSCP00000028556

UniGene: Ssc.16043

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.