Canine C-Reactive Protein,CRP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬C反应蛋白(CRP)酶联免疫试剂盒（CSB-E14262c）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的CRP含量。CRP即C反应蛋白，是一种急性时相反应蛋白。其水平升高与体内炎症反应和组织损伤密切相关。研究机制上，它能激活补体系统、调节免疫细胞功能等。在临床中常作为炎症和疾病活动的重要标志物，广泛用于疾病的诊断和病情监测。试剂盒检测范围为0.312 μg/mL-20 μg/mL，该产品适用于科研领域中对犬类炎症相关疾病模型的机制研究、免疫调控实验的效应评估，以及药物或疫苗开发中炎症指标的动态监测，为实验动物健康状态分析提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

CRP
Uniprot No.：

T2KEN6
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates
检测范围：

0.312 μg/mL-20 μg/mL
灵敏度：

0.078 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-103
1:2	Average %	89
	Range %	84-96
1:4	Average %	97
	Range %	90-108
1:8	Average %	89
	Range %	84-95

回收率：

The recovery of canine CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	92	87-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.