Dog growth hormone,GH ELISA Kit

Dog growth hormone,GH ELISA Kit

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中文名称：

犬生长激素(GH)酶联免疫试剂盒
货号：

CSB-E14962c
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

犬生长激素(GH)酶联免疫试剂盒（CSB-E14962c）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GH1含量。GH1 即生长激素 1 基因，它编码生长激素，在人体生长发育、代谢等生理过程中扮演重要角色。研究机制方面，主要围绕其调控生长激素合成与分泌、与生长信号通路的相互作用等，这些研究有助于深入理解生长发育异常机制及相关疾病治疗。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品适用于动物生长机制研究、代谢调控分析、药物或营养干预实验等科研领域，为探究犬类生理病理过程中的GH动态变化提供稳定工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

GH1 ELISA Kit; GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
缩写：

GH1
Uniprot No.：

P33711
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-97
1:2	Average %	98
	Range %	91-105
1:4	Average %	89
	Range %	84-95
1:8	Average %	99
	Range %	92-106

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.853

2.734

2.794

2.632

2.087

2.067

2.077

1.916

1.504

1.498

1.501

1.339

0.897

0.929

0.913

0.751

2.5

0.550

0.521

0.536

0.374

1.25

0.383

0.363

0.373

0.211

0.625

0.271

0.284

0.277

0.116

0.158

0.165

0.162

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Age-specific variations in hematological and biochemical parameters in middle-and large-sized of dogs SH Lee,J Vet Sci,2019

功能：

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
基因功能参考文献：
1. Progesterone alone has no direct stimulatory effect on GH transcription in mammary gland. PMID: 18262383
2. Study provides evidence that progesteron might increase autocrine Growth Hormone production which might directly stimulate local or systemic IGF-I secretion in canine mammary tumours. PMID: 18367392
亚细胞定位：

Secreted.
蛋白家族：

Somatotropin/prolactin family
数据库链接：

KEGG: cfa:403795

STRING: 9615.ENSCAFP00000018659

UniGene: Cfa.3656

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.