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Goat Thyroxine(T4) ELISA Kit

  • 中文名称:
    山羊甲状腺素(T4)酶联免疫试剂盒
  • 货号:
    CSB-E13384G
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    山羊甲状腺素(T4)酶联免疫试剂盒(CSB-E13384G)为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的T4含量。试剂盒检测范围为20 ng/mL-320 ng/mL,灵敏度为20 ng/mL。适用于科研领域中对山羊或其他偶蹄目动物甲状腺功能的动态监测,例如在营养代谢研究、环境内分泌干扰物评估、激素替代治疗模型构建中提供定量数据支持,亦可应用于畜牧学科研中饲料添加剂对甲状腺轴影响的药效学分析。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    T4
  • 种属:
    Capra hircus (Goat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    20 ng/mL-320 ng/mL
  • 灵敏度:
    20 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of goat T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 86-101    
    1:2 Average % 86    
    Range % 81-98    
    1:4 Average % 95    
    Range % 90-100    
    1:8 Average % 95    
    Range % 90-100    
  • 回收率:
    The recovery of goat T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 97 92-110    
    EDTA plasma (n=4) 95 90-105    
                   
                   
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average      
    320 0.163 0.169 0.166      
    160 0.275 0.281 0.278      
    80 0.493 0.502 0.498      
    40 1.015 1.029 1.022      
    20 2.049 2.068 2.059      
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

相关问答

 常见问题解答
Q:

I'm studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your inquiry. We have the mentioned kit available.For example:
#CSB-E13384G,Goat Thyroxine(T4) ELISA Kit *96T (If duplicate done,22 kits is advised;If not,only 11 kits is ok)
The standard wells are S1-S5 and duplicate is advised.With a blank well,the number of standard wells is 5×2+1=11.The rest is for samples wells,85=96-11(If duplicate is done for sample,then the number will be the half of that.)
If you want to detect diary goat samples,we suggest you do pre-testing first.

靶点详情

  • 最新研究进展:
    T4是一种重要的代谢激素,最新研究表明T4与身体免疫系统密切相关。研究人员正在探索T4对免疫细胞功能的影响,以及利用T4来治疗免疫系统相关疾病的可能性。