Rat High density lipoprotein cholesterol, HDL-C ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠高密度脂蛋白胆固醇（HDL-C）定量检测专用ELISA试剂盒（货号：CSB-E14399r），采用双抗体夹心法原理设计，可精准检测血清、血浆及组织匀浆样本中HDL-C含量，检测灵敏度覆盖93.75-6000 ng/mL的宽泛线性范围。实验通过预包被于微孔板的特异性捕获抗体结合样本中的HDL-C，再通过生物素标记的检测抗体与酶标链霉亲和素系统实现信号放大，最终通过显色反应强度进行定量分析。HDL-C作为重要的脂代谢指标，其水平与心血管健康密切相关，该试剂盒适用于动脉粥样硬化发病机制研究、脂代谢调控实验、药物干预效果评价等科研领域，可支持动物模型构建、营养学实验及心血管相关分子通路的探索。试剂盒包含预包被板、标准品、检测抗体及全套实验缓冲液，提供96孔板规格，操作流程标准化，具备良好的批内批间重复性，满足实验室对微量样本的高通量检测需求。
缩写：

HDL-C
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

93.75 ng/mL-6000 ng/mL
灵敏度：

23.4 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	87
	Range %	83-89
1:400	Average %	99
	Range %	95-102
1:800	Average %	90
	Range %	85-93
1:1600	Average %	99
	Range %	92-102

回收率：

The recovery of rat HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	107	101-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.