Rat tissue factor(TF)/coagulation factor VII(F7) complex ELISA kit

产品详情

产品描述：

本产品是一款专用于定量检测大鼠血清、血浆及组织匀浆样本中组织因子与活化凝血因子7复合物（TF/FVIIa）的ELISA试剂盒（货号：CSB-EQ027252RA），该复合物在凝血级联反应中发挥关键作用，参与血栓形成、炎症调控及细胞信号传导等生理病理过程。试剂盒采用双抗体夹心法原理，通过高特异性抗体对靶标进行捕获和检测，灵敏度达15.6 pg/mL，检测线性范围为15.6-1000 pg/mL，可精准分析不同浓度样本。适用于心血管疾病、血栓性疾病等动物模型的机制研究，或抗凝药物、靶向治疗方案的体外效果评估。试剂盒提供预包被96孔板及标准化试剂，操作流程简便，兼容常规实验室设备，实验全程约需4.5小时，为凝血相关基础研究提供可靠工具。本产品严格限定于科学研究用途，实验数据需结合其他生物学方法进行综合分析。
缩写：

TF
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TF/F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	98-110
1:2	Average %	103
	Range %	96-107
1:4	Average %	98
	Range %	89-102
1:8	Average %	105
	Range %	98-110

回收率：

The recovery of rat TF/F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	102	95-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.