Rat activated coagulation factor X(FXa) ELISA Kit

产品详情

产品描述：

CUSABIO大鼠活化凝血因子X（FXa）定量检测提供高效解决方案，适用于血清、血浆及组织匀浆样本的科研分析。FXa作为凝血级联反应的核心蛋白酶，通过催化凝血酶原转化为凝血酶参与止血与血栓形成调控，其活性变化与心血管疾病、炎症反应及抗凝机制研究密切相关。试剂盒采用竞争法检测原理，可实现15.6-1000 pg/mL范围内的精准定量，最低检测限达6.1 pg/mL，具备优异灵敏度与特异性。预包被96孔板提供即用型实验体系，操作流程包含标准品稀释、样本孵育、显色反应等步骤，3.5小时内即可完成检测。试剂盒包含全套优化组分，涵盖标准品、检测抗体、显色底物及终止液，支持实验室开展凝血机制研究、抗凝药物筛选、病理模型评估等方向实验。多样本兼容性设计尤其适合系统性研究凝血因子在组织微环境中的动态变化，为探索血栓性疾病、术后凝血异常等课题提供可靠工具。所有组分均经严格质控，确保批间稳定性，实验数据可支持ELISA分析仪在450nm波长下的定量读取。
缩写：

FXa
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	102
	Range %	98-109
1:400	Average %	90
	Range %	86-94
1:800	Average %	97
	Range %	92-101

回收率：

The recovery of rat FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	85-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.