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  4. Rat Cystatin C,Cys-C ELISA Kit

Rat Cystatin C,Cys-C ELISA Kit

文献引用 (23) 说明书下载
  • 中文名称：
    大鼠半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)酶联免疫试剂盒
  • 货号：
    CSB-E08385r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专为大鼠样本设计的半胱氨酸蛋白酶抑制剂/胱抑素C（Cys-C）定量检测试剂盒（货号：CSB-E08385r），采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆中的Cys-C蛋白浓度。Cys-C是一种广泛存在于体液中的半胱氨酸蛋白酶抑制剂，通过调控半胱氨酸蛋白酶活性参与细胞外基质代谢、炎症反应等生理病理过程，在肾脏功能评估和疾病机制研究中具有重要价值。该试剂盒检测范围为0.312-20 ng/ml，提供高灵敏度和特异性，适用于科研领域中对肾脏疾病模型、药物代谢研究或组织损伤相关实验中Cys-C表达水平的动态监测，帮助研究者分析其与肾功能状态、炎症进程或细胞凋亡的关联性。实验样本兼容液体类（血清、血浆）及匀浆组织处理液，操作流程标准化，数据稳定可靠，满足实验室对生物标志物定量分析的需求。
  • 别名：
    Cst3 ELISA Kit; Cystatin-C ELISA Kit; Cystatin-3 ELISA Kit
  • 缩写：
    CST3
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, homogenates
  • 检测范围：
    0.312 ng/ml-20 ng/ml
  • 灵敏度：
    0.078 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

    　

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    2.490

    2.550

    2.588

    2.467

    2.527

    2.555

    SD

    0.031

    0.039

    0.046

    0.041

    0.048

    0.056

    CV(%)

    3.407

    4.225

    4.941

    4.530

    5.229

    6.061

  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:2

    Average %

    96

    Range %

    93-99

    1:4

    Average %

    93

    Range %

    90-96

    1:8

    Average %

    96

    Range %

    91-104

    1:16

    Average %

    92

    Range %

    89-95

  • 回收率：

    The recovery of rat Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    93

    89-97

    EDTA plasma (n=4)

    94

    90-98

  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    C:UsersAdministratorAppDataLocalTemp企业微信截图_17157650865552.png

    ng/ml

    OD1

    OD2

    Average

    Corrected

    20

    2.564

    2.587

    2.576

    2.404

    10

    1.923

    1.934

    1.929

    1.757

    5

    1.329

    1.368

    1.349

    1.177

    2.5

    0.912

    0.937

    0.925

    0.753

    1.25

    0.582

    0.598

    0.590

    0.418

    0.625

    0.412

    0.436

    0.424

    0.252

    0.312

    0.311

    0.317

    0.349

    0.177

    0

    0.170

    0.174

    0.172

    　

  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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Cst3 Proteins

靶点详情

  • 最新研究进展：
    CST3是一种由人体细胞产生的小分子蛋白质，也被称为半胱氨酸蛋白酶抑制剂或淀粉样蛋白沉积物抑制剂。它在细胞凋亡、免疫调节、组织修复和生殖系统发育等方面发挥重要作用。最近的研究表明，CST3与多种疾病有关，包括神经退行性疾病、肾脏疾病和肿瘤。
     
  • 功能：
    As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.
  • 基因功能参考文献：
    1. Tanshinone IIA protects against cardiac hypertrophy in spontaneously hypertensive rats by inhibiting the Cys-C/Wnt signaling pathway. PMID: 28053285
    2. These results indicate that Cd increases the urinary excretion of cystatin C, and they suggest that this effect may involve disruption of megalin-mediated uptake of cystatin C by epithelial cells of the proximal tubule. PMID: 26715107
    3. High cystatin C is associated with proteinuria. PMID: 21551085
    4. Triiodothyronine-induced increase in the production of cystatin C may be related to an increased cell metabolism and proteolysis control demand. PMID: 22360852
    5. CysC plays a protective role under conditions of neuronal challenge by inducing autophagy via mTOR inhibition PMID: 20352108
    6. Oxidants induce elevated cystatin C production from cardiac myocytes. Cystatin C plays a role in cardiac extracellular matrix remodelling. PMID: 20489058
    7. We found several novel glycoforms of cystatin c in rat hippocampal stem cells, suggesting that N- and O-linked glycans with sialic acid are attached to cystatin C. PMID: 15197734
    8. These results demonstrate that cystatin C has protective effects against various oxidative stresses that induce cell death. PMID: 16140167
    9. endometrial expression of the cysteine protease inhibitor, Cst3, is modulated by estrogen during the peri-implantation period PMID: 16935259
    10. These studies suggest alternate pathways and potential therapeutic targets of cell death associated with oxidative stress, CC, and lysosomal dysregulation. PMID: 17241700
    11. Finally, in vivo studies using kidney-specific megalin knockout mice provide evidence that megalin mediates proximal tubular uptake of cystatin C. We conclude that megalin is an endocytic receptor of cystatin C in PTC. PMID: 17462596

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Cystatin family
  • 数据库链接：

    KEGG: rno:25307

    STRING: 10116.ENSRNOP00000007175

    UniGene: Rn.106351

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