Rat Coagulation factor VII(F7) ELISA kit

产品详情

产品描述：

大鼠凝血因子VII(F7)酶联免疫试剂盒（CSB-EL007930RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的F7含量。大鼠凝血因子VII（F7）是一种依赖维生素K的丝氨酸蛋白酶，在血液凝固的外部途径中起关键作用。其与组织因子III（TF III）结合后，形成TF-FVIIa激活复合物，启动凝血级联反应，最终导致纤维蛋白凝块形成。F7缺乏会导致围产期死亡，因为其完全缺乏会导致正常血管中的出血。试剂盒检测范围为0.78 ng/mL-50 ng/mL，其水平变化与凝血功能异常、肝损伤及心血管疾病模型研究密切相关；支持科研领域对凝血机制、药物作用靶点筛选、肝脏功能评估等研究需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

F7Coagulation factor VII ELISA Kit; EC 3.4.21.21 ELISA Kit; Serum prothrombin conversion accelerator) [Cleaved into: Factor VII light chain; Factor VII heavy chain] ELISA Kit
缩写：

F7
Uniprot No.：

Q8K3U6
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Blood Coagulation
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-98
1:400	Average %	97
	Range %	92-106
1:800	Average %	93
	Range %	88-99
1:1600	Average %	89
	Range %	83-95

回收率：

The recovery of rat F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	109	103-115

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
基因功能参考文献：
1. The EGF(1) region of rat coagulation factor can mediate the specific binding of FVII with tissue factor. PMID: 18315926
亚细胞定位：

Secreted.
蛋白家族：

Peptidase S1 family
组织特异性：

Plasma.
数据库链接：

KEGG: rno:260320

STRING: 10116.ENSRNOP00000038466

UniGene: Rn.86416