Human Vascular Endothelial Growth Factor 121(VEGF121) ELISA kit

Human Vascular Endothelial Growth Factor 121(VEGF121) ELISA kit

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中文名称：

人血管内皮生长因子121(VEGF121)酶联免疫试剂盒
货号：

CSB-E13709h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO试剂盒采用双抗体夹心法定量检测人血管内皮生长因子121（VEGF121），该蛋白是血管内皮生长因子家族的重要成员，具有促进血管生成和调控内皮细胞增殖的关键作用，其可溶性特性使其在肿瘤微环境、心血管疾病及组织修复等科研领域备受关注。试剂盒适用于血清、血浆、细胞培养上清及组织匀浆样本检测，灵敏度下限为0.156 ng/mL，线性范围覆盖至10 ng/mL，可精准量化低丰度样本中的目标蛋白。实验采用预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，配套提供标准品、检测缓冲液及显色底物等完整组分。该产品广泛应用于肿瘤血管生成机制研究、心血管疾病模型评估、再生医学实验等科研场景，其高灵敏度和多样本兼容性为体外实验、动物模型及细胞水平的研究提供可靠的数据支持，实验全程可在4-6小时内完成，便于实验室高效开展VEGF121相关的分子机制探索。
缩写：

VEGF121
Uniprot No.：

P15692
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF121 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-106
1:2	Average %	94
	Range %	90-98
1:4	Average %	89
	Range %	86-96
1:8	Average %	92
	Range %	86-97

回收率：

The recovery of human VEGF121 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-104
EDTA plasma (n=4)	91	87-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.415	2.314	2.365	2.222
5	1.532	1.633	1.583	1.440
2.5	0.972	0.951	0.962	0.819
1.25	0.558	0.547	0.553	0.410
0.625	0.404	0.411	0.408	0.265
0.312	0.285	0.274	0.280	0.137
0.156	0.226	0.220	0.223	0.080
0	0.143	0.142	0.143

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
相关疾病：

Microvascular complications of diabetes 1 (MVCD1)
亚细胞定位：

Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a significant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
蛋白家族：

PDGF/VEGF growth factor family
组织特异性：

Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.
数据库链接：

HGNC: 12680

OMIM: 192240

KEGG: hsa:7422

STRING: 9606.ENSP00000361125

UniGene: Hs.73793

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.