Human pyridinoline (PYD) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫法，专用于定量检测人血清、血浆及尿液样本中胶原吡啶交联（PYYD）的含量。PYD作为I型胶原蛋白特异性降解产物，是反映骨吸收过程的重要生物标志物，其水平变化与骨质疏松、类风湿性关节炎等骨代谢疾病密切相关。检测体系覆盖12.5至800 ng/mL的宽泛线性范围，通过预包被捕获抗体与样本中PYD特异性结合，经生物素化检测抗体二次识别后形成复合物，最终通过酶标仪读取显色信号实现精确定量。试剂盒包含预包被96孔板、梯度浓度标准品、生物素标记检测抗体及显色底物等完整组分，适用于骨代谢机制研究、抗骨质疏松药物筛选、关节炎动物模型评估等科研领域。特别针对尿液样本的兼容性设计，为无创性骨代谢动态监测提供了便捷方案，可满足体外培养体系检测、疾病模型纵向观察等实验需求，配套的优化缓冲体系确保在复杂生物基质中仍保持优异检测性能。
别名：

N/A
缩写：

PYD
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PYD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-100
1:2	Average %	92
	Range %	86-99
1:4	Average %	87
	Range %	80-94
1:8	Average %	97
	Range %	92-102

回收率：

The recovery of human PYD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	85-99
EDTA plasma (n=4)	98	92-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.