Human activated coagulation factor Ⅻ,FⅫa ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人活化凝血因子Ⅻ（FⅫa）酶联免疫检测试剂盒（货号：CSB-E09232h），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的FⅫa浓度，检测灵敏度达0.312 ng/mL，线性范围为0.312-20 ng/mL。FⅫa是接触激活系统的核心蛋白酶，在凝血级联反应及炎症调节中发挥关键作用，其异常活化与血栓性疾病、遗传性血管性水肿等病理过程密切相关。本试剂盒通过高特异性抗体对靶标进行捕获与检测，适用于体外研究FⅫa在凝血机制、血管通透性调控或相关疾病动物模型中的动态变化，可为心血管疾病、免疫炎症反应等领域的科研实验提供定量分析支持。实验操作便捷，兼容常规实验室设备，所有组分均经过严格验证，确保结果的稳定性和重复性。该产品仅用于科学研究，不适用于其他用途。
缩写：

FⅫa
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FⅫa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	111
	Range %	105-116
1:2	Average %	95
	Range %	88-102
1:4	Average %	96
	Range %	90-100
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of human FⅫa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
EDTA plasma (n=4)	108	103-114

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.