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  4. Recombinant Arabidopsis thaliana Omega-3 fatty acid desaturase, endoplasmic reticulum (FAD3)

Recombinant Arabidopsis thaliana Omega-3 fatty acid desaturase, endoplasmic reticulum (FAD3)

  • 中文名称：
    拟南芥FAD3重组蛋白
  • 货号：
    CSB-CF342958DOA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    FAD3
  • Uniprot No.：
    P48623
  • 别名：
    FAD3; At2g29980; F23F1.10; Acyl-lipid omega-3 desaturase; cytochrome b5, endoplasmic reticulum; Omega-3 fatty acid desaturase 3, endoplasmic reticulum
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-386
  • 氨基酸序列
    MVVAMDQRTNVNGDPGAGDRKKEERFDPSAQPPFKIGDIRAAIPKHCWVKSPLRSMSYVV RDIIAVAALAIAAVYVDSWFLWPLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGH ILHSFILVPYHGWRISHRTHHQNHGHVENDESWVPLPERVYKKLPHSTRMLRYTVPLPML AYPLYLCYRSPGKEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVL KVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNNIHHD IGTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYV SDTGDIVFYETDPDLYVYASDKSKIN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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FAD3 Proteins

靶点详情

  • 功能：
    Microsomal (ER) omega-3 fatty acid desaturase introduces the third double bond in the biosynthesis of 18:3 fatty acids, important constituents of plant membranes. It is thought to use cytochrome b5 as an electron donor and to act on fatty acids esterified to phosphatidylcholine and, possibly, other phospholipids.
  • 基因功能参考文献：
    1. Histone acetyltransferase general control non-repressed protein 5 (GCN5) affects the fatty acid composition of Arabidopsis thaliana seeds by acetylating fatty acid desaturase3 (FAD3). PMID: 27500884
    2. ALA10 interacts with FATTY ACID DESATURASE2 (FAD2), and modification of ALA10 expression affects phosphatidylcholine (PC) fatty acyl desaturation by disturbing the balance between FAD2 and FAD3 activities. PMID: 26620528
    3. SAD6 functions as a Delta9-desaturase, and together with FAD3 it increases the levels of unsaturated fatty acids in crown galls under hypoxia and drought stress conditions. PMID: 24368335
    4. FAD2 and FAD3 desaturases form heterodimers that facilitate metabolic channel. PMID: 24811169
    5. that bZIP67 binds G-boxes in the FAD3 promoter and enhances FAD3 expression. PMID: 23995083
    6. Increase in the dosage of FAD3 results in an increase in the linolenic acid content of seed oil. PMID: 21848868
    7. The fatty acid composition of membrane lipids influences cell growth and mitochondrial respiration, in particular the expression and capacity of alternative oxidase, under cold stress in fad2 knockout and FDAD3 overexpressing cell lines PMID: 17507388
    8. The polymethylene-interrupted dienoic fatty acid, mangiferic acid, can be produced in seed oil through the overexpression of a fatty acid n-3 desaturase. PMID: 19548018

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Fatty acid desaturase type 1 family
  • 组织特异性：
    Abundant in leaves and seedlings. Barely detectable in root tissue.
  • 数据库链接：

    KEGG: ath:AT2G29980

    STRING: 3702.AT2G29980.1

    UniGene: At.271

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