Recombinant Arabidopsis thaliana L-galactono-1,4-lactone dehydrogenase, mitochondrial (GLDH)

in vitro E.coli expression system

中文名称：

拟南芥GLDH重组蛋白
货号：

CSB-CF890441DOA
规格：
来源：

in vitro E.coli expression system
其他：

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基因名：

GLDH
Uniprot No.：

Q9SU56
别名：

GLDH; At3g47930; T17F15.200; L-galactono-1,4-lactone dehydrogenase, mitochondrial
种属：

Arabidopsis thaliana (Mouse-ear cress)
蛋白长度：

Full Length of Mature Protein
表达区域：

102-610
氨基酸序列

YAPLPEDLHTVSNWSGTHEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGI GLSRSGMVNLALMDKVLEVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIG GIIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAE VTLQCVARHELVEHTYVSNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWS GPPKDKPKYTTDEAVQHVRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLA LDPLNDVHVAKVNQAEAEFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSM KDLEYIEELKKLIEKEAIPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTA DPRQRKDITDEFFHYRHLTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDA YNKARRELDPNRILSNNMVEKLFPVSTTA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 10xHis-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Involved in the biosynthesis of ascorbic acid. Required for the accumulation of respiratory complex I. Uses L-galactono-1,4-lactone and L-gulono-1,4-lactone as substrates, but not D-galactono-1,4-lactone, D-gulono-1,4-lactone, L-mannono-1,4-lactone or D-galactonic acid. Also active with phenazine methosulfate and 1,4-benzoquinone as electron acceptors.
基因功能参考文献：
1. GLDH is a dual-function protein that has a second, non-enzymatic function in complex I assembly as a plant-specific assembly factor. PMID: 26520835
2. a role of GLDH during complex I formation, which is based on its binding to specific assembly intermediates. PMID: 22378782
3. Recombinant GALDH from Arabidopsis thaliana is inactivated by hydrogen peroxide due to selective oxidation of cysteine (Cys)-340, located in the cap domain. [GALDH] PMID: 19369590
4. Data show that AtGALDH forms a flavin N5 sulfite adduct and that AtGALDH lacks the histidine involved in covalent FAD binding, but contains a leucine instead. PMID: 18190525
5. L-galactono-1,4-lactone dehydrogenase is required for the accumulation of respiratory complex I PMID: 18799460
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亚细胞定位：

Mitochondrion membrane; Single-pass membrane protein.
数据库链接：

KEGG: ath:AT3G47930

STRING: 3702.AT3G47930.1

UniGene: At.1530

Recombinant Arabidopsis thaliana L-galactono-1,4-lactone dehydrogenase, mitochondrial (GLDH)

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