V5-Tag Monoclonal Antibody

中文名称：

V5-Tag鼠单克隆抗体
货号：

CSB-MA000161M1m
规格：

￥400
图片：
Western Blot
Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, 5, 6 at 50ng
All lanes: V5 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31.9, 20.4, 55, 24.2, 53.4, 30 KDa
Observed band size: 32, 21, 55, 28, 58, 33 KDa
Exposure time: 5min

Western Blot
Positive WB detected in: 50ng V5-tagged fusion protein
V5 Tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 32 KDa
Observed band size: 32 KDa
Exposure time: 5min

Western Blot
Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:2000
Lane 1: Recombinant V5-tagged fusion protein at 25ng
Lane 2: Recombinant V5-tagged fusion protein at 12.5ng
Lane 3: Recombinant V5-tagged fusion protein at 6.25ng
Lane 4: Recombinant V5-tagged fusion protein at 3.125ng
Lane 5: Recombinant V5-tagged fusion protein at 1.56ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 32 kd
Observed band size: 32 kd
Exposure time: 5min

Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).

Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
Lane 1: Mouse control IgG2a instead of CSB-MA000161M1m in Transfected HEK293 cells whole cell lysate
Lane 2: CSB-MA000161M1m (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg)
Lane 3: Transfected HEK293F cells whole cell lysate (20µg)
For western blotting, the blot was detected with CSB-MA000161M1m at 1:3000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
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CUSABIO货号：CSB-MA000161M1m V5-Tag单克隆抗体是一款针对V5表位标签设计的高特异性科研试剂，适用于检测重组蛋白中的V5标签。V5-Tag作为分子生物学研究中常用的短肽标签（14个氨基酸），可特异性标记重组蛋白以便于追踪、纯化和功能研究。本产品经严格验证具有卓越的物种广谱性，可在ELISA实验中实现精准定量检测，通过Western Bloting（WB）清晰呈现目标条带，并支持免疫荧光（IF）进行亚细胞定位分析，同时适用于免疫共沉淀（IP）实验以研究蛋白质相互作用。抗体采用杂交瘤技术制备，批次稳定性优异，可满足基因表达调控、蛋白质相互作用网络构建、转基因生物模型验证等科研需求。该产品兼容哺乳动物、昆虫等多种表达系统，是研究重组蛋白表达效率、亚细胞定位及功能机制的理想工具，广泛应用于分子生物学、细胞生物学及蛋白质组学领域的基础研究。
基因名：

V5-Tag
宿主：

Mouse
反应种属：

All
免疫原：

GKPIPNPLLGLDST synthetic peptide conjugate to KLH
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

IgG2a
纯化方式：

>95%, Protein A purified
克隆号：

9E8B7
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB, IF, IP
推荐稀释比：

Application Recommended Dilution

WB 1:5000-1:1280000

IF 1:100-1:200

IP 2µl-5µl
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

V5-Tag Monoclonal Antibody

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Application	Recommended Dilution
WB	1:5000-1:1280000
IF	1:100-1:200
IP	2µl-5µl