Avi-Tag Monoclonal Antibody

中文名称：

Avi-Tag鼠单克隆抗体
货号：

CSB-MA000101M0m
规格：

￥400
图片：
Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli and Mammalian cell All lanes: Avi tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40, 35, 30, 70, 40, 33, 35, 40 KDa
Observed band size: 40, 35, 30, 70, 40, 33, 35, 4 KDa
Exposure time：1min

Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli All lanes: Avi tag antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 81.2, 67, 66.9, 66.9 KDa
Observed band size: 82, 67, 67, 67KDa
Exposure time：5min

Western Blot
Positive WB detected in: Recombinant protein from Mammalian cell with Avi tag from E.coli at 100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: Avi tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40 KDa
Observed band size: 40 KDa
Exposure time：1min

Western Blot
Positive WB detected in: 50ng Recombinant protein with Avi tag from Mammalian cell Avi tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40 KDa
Observed band size: 40 KDa
Exposure time：5min

Immunoprecipitating Avi tag in 293F whole cell lysate transfected with avi tag
Lane 1: Mouse control IgG instead of CSB-MA000101M0m in 293F whole cell lysate transfected with avi tag. Lane 2: CSB-MA000101M0m (1µg) + 293F whole cell lysate transfected with avi tag (500µg)
Lane 3: 293F whole cell lysate transfected with avi tag(40µg)
For western blotting, the blot was detected with CSB-MA000101M0m at 1:1000, and a HRP-conjugated Goat anti Mouse antibody was used as the secondary antibody at 1:50000
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产品描述：

CUSABIO货号：CSB-MA000101M0m Avi-Tag单克隆抗体是针对生物素化标签系统开发的高特异性科研试剂，其靶向的AVI标签是广泛应用于重组蛋白标记领域的15个氨基酸短肽序列，通过与链霉亲和素的高亲和力结合，显著提升蛋白纯化效率及检测灵敏度。本产品采用杂交瘤技术制备，具备严格的批间一致性，经实验验证可特异性识别AVI标签而不与天然生物素发生交叉反应，适用于ELISA、Western Blot和免疫沉淀（IP）三大核心蛋白研究技术平台。在重组蛋白表达验证实验中，该抗体能有效检测哺乳动物、昆虫等多种表达系统产生的AVI标记蛋白；在蛋白互作研究中，可通过IP实验高效富集标记蛋白复合物；其Western Blot应用可精准识别1-100 kDa分子量范围内的标记靶蛋白。该抗体已通过多种物种样本验证，为基因编辑验证、蛋白互作组学研究和体外诊断试剂开发提供关键工具，特别适用于需要高灵敏度检测的生物制药研发、蛋白质工程优化等生命科学领域。本产品严格遵循科研试剂标准，配套提供详细技术参数及实验方案，助力研究人员实现精准高效的AVI标记蛋白检测与功能解析。
产品名称：

Mouse anti-All AVI Monoclonal antibody
基因名：

AVI
宿主：

Mouse
反应种属：

All
免疫原：

Synthetic Peptide
免疫原种属：

All
标记方式：

Non-conjugated
克隆类型：

Monoclonal
抗体亚型：

IgG2a
纯化方式：

>95%, Protein G purified
克隆号：

9G3B5
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB, IP
推荐稀释比：

Application Recommended Dilution

WB 1:1000-1:640000

IP 2µl-5µl
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunoprecipitation (IP) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Avi-Tag Monoclonal Antibody

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Application	Recommended Dilution
WB	1:1000-1:640000
IP	2µl-5µl